Ariyanti Tati, Suhaemi Suhaemi, Mulyati Sri, Sukatma Sukatma, Sumirah Sumirah, Noor Susan Maphilindawati, Rachmawati Faidah, Widiyanti Prima Mei, Sukmawinata Eddy, Andriani Andriani, Kusumaningtyas Eni, Khairullah Aswin Rafif
Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Bogor, Indonesia.
Open Vet J. 2025 Mar;15(3):1340-1348. doi: 10.5455/OVJ.2025.v15.i3.25. Epub 2025 Mar 31.
The alarming rise in infections caused by extended-spectrum beta-lactamase (ESBL)-producing in animals and humans poses a serious threat due to its escalating antibiotic resistance. Unveiling this problematic bacteria's prevalence and resistance patterns in animals is crucial for formulating effective control strategies and safeguarding public health.
The purpose of this study was to analyze the expression of three main genes: CTX-M, SHV, and TEM, in ESBL-producing isolates from The Research Center for Veterinary Science and the National Research and Innovation Agency. Moreover, their resistance profiles against various antibiotics should be systematically evaluated.
Ninety-seven isolates from the bacteriology laboratory of The Research Center for Veterinary Science were identified on MacConkey medium supplemented with cefotaxime. The isolates were verified for the existence of the CTX-M, SHV, and TEM genes using PCR. Antimicrobial susceptibility testing was conducted using antibiotic discs following the CLSI standards.
The prevalence of ESBL-producing in chicken ceca, eggs, and fish intestines was 16.5% (16/97). The specific genes detected were CTX-M gene at 93.75% (15/16), followed by the TEM gene, at 81.25% (13/16), and blaSHV at 25% (4/16). The antimicrobial sensitivity test results revealed that all ESBL-producing isolates had multidrug resistance 81.25% to 1-5 antibiotics and 18.75% to 6-7 antibiotics. The isolate exhibited 100% resistance to ampicillin and sulfamethoxazole, with exclusive sensitivity to chloramphenicol.
The dominant gene in the ESBL-producing isolates was CTX-M. This bacterium is completely resistant to ampicillin and sulfamethoxazole, whereas it displays multidrug resistance to 1-7 different types of antibiotics.
产超广谱β-内酰胺酶（ESBL）的细菌在动物和人类中引起的感染激增，因其不断增强的抗生素耐药性构成了严重威胁。揭示这种问题细菌在动物中的流行情况和耐药模式对于制定有效的控制策略和保障公众健康至关重要。
本研究的目的是分析来自兽医科学研究中心和国家研究与创新机构的产ESBL细菌分离株中三个主要基因CTX-M、SHV和TEM的表达情况。此外，还应系统评估它们对各种抗生素的耐药谱。
在补充头孢噻肟的麦康凯培养基上对来自兽医科学研究中心细菌学实验室的97株细菌分离株进行鉴定。使用聚合酶链反应（PCR）验证分离株中CTX-M、SHV和TEM基因的存在。按照美国临床和实验室标准协会（CLSI）标准，使用抗生素纸片进行药敏试验。
在鸡盲肠、鸡蛋和鱼肠道中产ESBL细菌的流行率为16.5%（16/97）。检测到的特定基因中，CTX-M基因占93.75%（15/16），其次是TEM基因，占81.25%（13/16），blaSHV基因占25%（4/16）。药敏试验结果显示，所有产ESBL细菌分离株均具有多重耐药性，81.25%对1至5种抗生素耐药，18.75%对6至7种抗生素耐药。该分离株对氨苄西林和磺胺甲恶唑表现出100%耐药，对氯霉素仅敏感。
产ESBL细菌分离株中的主导基因是CTX-M。这种细菌对氨苄西林和磺胺甲恶唑完全耐药，而对1至7种不同类型的抗生素表现出多重耐药。
