Sordillo P P, Andreeff M, Helson L, Steinmetz J, Melamed M R
Cancer Invest. 1985;3(4):321-5. doi: 10.3109/07357908509039794.
Studies were carried out on the effect of dianhydrogalactitol on a human neuroblastoma cell line in culture. The drug was cytotoxic at concentrations of 5 micrograms/ml and 10 micrograms/ml for 1 hour, but not at 1 microgram/ml. Flow cytometry of DNA showed that cell kill was preceded by a transient accumulation of cells in early S at 10 hours, and in late S at 36 hours. Smaller increases in the percent of cells in S phase were seen after treatment with 1 microgram/ml, without cell kill. It may be concluded that dianhydrogalactitol causes an S-phase block, followed by death of cells in late S at concentrations above 5 micrograms/ml.
开展了关于双脱水半乳糖醇对培养的人神经母细胞瘤细胞系作用的研究。该药物在浓度为5微克/毫升和10微克/毫升处理1小时时有细胞毒性，但在1微克/毫升时无细胞毒性。DNA流式细胞术显示，细胞死亡之前，在10小时时细胞在早S期短暂积累，在36小时时在晚S期短暂积累。用1微克/毫升处理后，S期细胞百分比有较小增加，但无细胞死亡。可以得出结论，双脱水半乳糖醇导致S期阻滞，在浓度高于5微克/毫升时，随后是晚S期细胞死亡。
