Institoris E, Fox B W
Chem Biol Interact. 1978 Jul;22(1):99-107. doi: 10.1016/0009-2797(78)90153-9.
Using alkaline sucrose gradient sedimentation centrifugation it was found that treatment of Yoshida sarcoma cells in culture for 1 h with increasing concentrations of dianhydrogalactitol (DAG) enhanced the sedimentation rate of DNA in a dose-dependent manner. There was no difference between the amount of protein which co-sedimented with DNA released from treated and untreated cells. When DNA was extracted from the cells using a p-amino-salicylate-phenol mixture, the protein content of DNA seemed not to be affected by DAG. The possibility that DAG could form interstrand cross-linking in cellular DNA was suggested from renaturation studies. The appearance of a fast sedimenting DNA in the alkaline sucrose gradient and the evidence for a cross-linked DNA detected by renaturation technique, only appeared later than 6 h after treatment. A similar delayed effect on the depression in the rate of DNA synthesis was also observed. These data suggest that the inhibition of DNA synthesis may be related to the delayed formation of DNA interstrand cross-linked.
采用碱性蔗糖梯度沉降离心法发现，用浓度递增的双脱水半乳糖醇（DAG）处理培养的吉田肉瘤细胞1小时，可使DNA的沉降速率呈剂量依赖性增加。处理过的细胞和未处理的细胞释放出的与DNA共沉降的蛋白量没有差异。当使用对氨基水杨酸 - 苯酚混合物从细胞中提取DNA时，DNA的蛋白质含量似乎不受DAG影响。复性研究表明DAG可能在细胞DNA中形成链间交联。碱性蔗糖梯度中快速沉降的DNA的出现以及复性技术检测到的交联DNA的证据，仅在处理后6小时后才出现。在DNA合成速率降低方面也观察到类似的延迟效应。这些数据表明DNA合成的抑制可能与DNA链间交联的延迟形成有关。
