Maxim Monica-Elisabeta, Toma Raluca-Marieta, Aricov Ludmila, Leonties Anca-Ruxandra, Precupas Aurica, Tatia Rodica, Oprita Elena Iulia
Romanian Academy, Ilie Murgulescu-Institute of Physical Chemistry, 202 Splaiul Independentei, 060021 Bucharest, Romania.
National Institute of Research and Development for Biological Sciences, 296 Splaiul Independentei, 060031 Bucharest, Romania.
Gels. 2025 Apr 16;11(4):294. doi: 10.3390/gels11040294.
For decades, royal jelly achieved notoriety and became an ultra-rich ingredient with numerous pharmacological properties especially for its use in production of topical ointments and creams. A novel formulation enriched with 2% royal jelly has been developed and characterized. Rheological results highlight a gel-like behavior of the product in the packaging, as it does not flow from the costumer's hand after application and behaves like a liquid, spreading evenly onto clean skin. A clear comparison in size distribution of pure and cream samples was noticed by dynamic light scattering analysis and completed further by Fourier transform infrared spectroscopy (FTIR-ATR) which showed off shift changes in the gel sample as compared to pure compounds. MTT assays were conducted in quintuplicate on murine fibroblasts cell line (NCTC L-929) for testing the biocompatibility of the product in the range of 50-1000 μg/mL over 24, 48 and 72 h. The designed formulation is typically intended to deliver active compounds to the skin surface and potentially into deeper layers. A molecular docking study was performed for binding mode prediction of P-gp protein residues with two ligands, quercetin and myricetin, in order to investigate their role in the internal modulation of drug transport across cell membranes within the skin.
几十年来，蜂王浆声名远扬，成为一种富含多种药理特性的超级成分，尤其在用于生产外用软膏和乳膏方面。一种富含2%蜂王浆的新型配方已被研发并表征。流变学结果表明该产品在包装中呈现出凝胶状特性，涂抹后不会从使用者手中流掉，且表现得像液体一样，能均匀地铺展在清洁的皮肤上。通过动态光散射分析可以明显看出纯样品和乳膏样品在尺寸分布上的差异，傅里叶变换红外光谱（FTIR-ATR）进一步完善了这一结果，该光谱显示与纯化合物相比，凝胶样品存在偏移变化。对小鼠成纤维细胞系（NCTC L-929）进行了五次MTT试验，以测试该产品在50 - 1000μg/mL浓度范围内、24小时、48小时和72小时的生物相容性。所设计的配方通常旨在将活性化合物递送至皮肤表面，并有可能渗透到更深层。为了研究槲皮素和杨梅素这两种配体在皮肤内药物跨细胞膜转运的内部调节中的作用，进行了分子对接研究，以预测P - gp蛋白残基与这两种配体的结合模式。
