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天然提取物、蜂蜜和蜂胶作为人类诺如病毒抑制剂。

Natural extracts, honey, and propolis as human norovirus inhibitors.

机构信息

Schaller Research Group at the University of Heidelberg and the DKFZ, Heidelberg, Germany.

Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany.

出版信息

Sci Rep. 2022 May 17;12(1):8116. doi: 10.1038/s41598-022-11643-5.

DOI:10.1038/s41598-022-11643-5
PMID:35581271
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9114320/
Abstract

Norovirus is the most important cause of acute gastroenteritis, yet there are still no antivirals, vaccines, or treatments available. Several studies have shown that norovirus-specific monoclonal antibodies, Nanobodies, and natural extracts might function as inhibitors. Therefore, the objective of this study was to determine the antiviral potential of additional natural extracts, honeys, and propolis samples. Norovirus GII.4 and GII.10 virus-like particles (VLPs) were treated with different natural samples and analyzed for their ability to block VLP binding to histo-blood group antigens (HBGAs), which are important norovirus co-factors. Of the 21 natural samples screened, date syrup and one propolis sample showed promising blocking potential. Dynamic light scattering indicated that VLPs treated with the date syrup and propolis caused particle aggregation, which was confirmed using electron microscopy. Several honey samples also showed weaker HBGA blocking potential. Taken together, our results found that natural samples might function as norovirus inhibitors.

摘要

诺如病毒是急性肠胃炎最重要的病因，但目前尚无抗病毒药物、疫苗或治疗方法。多项研究表明，诺如病毒特异性单克隆抗体、纳米抗体和天然提取物可能具有抑制作用。因此，本研究旨在确定其他天然提取物、蜂蜜和蜂胶样本的抗病毒潜力。用不同的天然样本处理诺如病毒 GII.4 和 GII.10 病毒样颗粒 (VLP)，并分析其阻断 VLP 与重要诺如病毒辅助因子——组织血型抗原 (HBGA) 结合的能力。在筛选的 21 种天然样本中，枣花蜜和一种蜂胶样本显示出有希望的阻断潜力。动态光散射表明，用枣花蜜和蜂胶处理的 VLP 导致颗粒聚集，这一结果通过电子显微镜得到了证实。一些蜂蜜样本也显示出较弱的 HBGA 阻断潜力。总之，我们的研究结果发现，天然样本可能具有抑制诺如病毒的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/6ec67f02d01c/41598_2022_11643_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/2a3fe959b608/41598_2022_11643_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/514efb3f1435/41598_2022_11643_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/a727e552a632/41598_2022_11643_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/e295b76dd722/41598_2022_11643_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/30b5fed3836d/41598_2022_11643_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/8f9ae00d313a/41598_2022_11643_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/b060e49761f0/41598_2022_11643_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/0bb6e3a9663d/41598_2022_11643_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/6ec67f02d01c/41598_2022_11643_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/2a3fe959b608/41598_2022_11643_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/514efb3f1435/41598_2022_11643_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/a727e552a632/41598_2022_11643_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/e295b76dd722/41598_2022_11643_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/30b5fed3836d/41598_2022_11643_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/8f9ae00d313a/41598_2022_11643_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/b060e49761f0/41598_2022_11643_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/0bb6e3a9663d/41598_2022_11643_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a6/9114320/6ec67f02d01c/41598_2022_11643_Fig9_HTML.jpg

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