收获的甜瓜接种后的全基因组DNA甲基化分析。

Whole-Genome DNA Methylation Analysis of Inoculation with in Harvested Muskmelons.

作者信息

Lyu Liang, Li Lei, Zhao Chenglong, Ning Yuchao, Luo Yawen, He Xining, Nan Mina

机构信息

College of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China.

Laboratory and Practice Base Management Center, Gansu Agricultural University, Lanzhou 730070, China.

出版信息

J Fungi (Basel). 2025 Mar 22;11(4):243. doi: 10.3390/jof11040243.

DOI:10.3390/jof11040243

PMID:40278064

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12027829/

Abstract

DNA methylation is a crucial epigenetic marker linked to plant defense responses, but its significance in fungal infection of postharvest fruits remains poorly understood. This study indicated that inoculation increased ROS production, enhanced phenylpropanoid metabolism-related enzyme activity, and promoted lignin accumulation in harvested muskmelon fruits cv. Yujinxiang) within 24 h post-inoculation (hpi). In addition, whole-genome bisulfite sequencing showed that genomic DNA methylation levels of muskmelon decreased by 6.15% at 24 hpi. Notably, CG sites exhibited a higher methylation level and the largest number of differentially methylated regions (DMRs). Moreover, 176 DMR-associated genes (DMGs) involved in the defense response, 134 DMGs in the ROS metabolic pathway, and 41 DMGs in phenylpropanoid metabolism were identified. The differentially expressed genes harboring differential methylation were mainly influenced by hypomethylation and exhibited elevated transcript levels, involved in phenylpropanoid biosynthesis and biosynthesis of secondary metabolites.

摘要

DNA甲基化是一种与植物防御反应相关的关键表观遗传标记，但其在采后果实真菌感染中的意义仍知之甚少。本研究表明，接种后24小时内，接种增加了采后甜瓜（品种：玉金香）果实中的活性氧生成，增强了苯丙烷类代谢相关酶的活性，并促进了木质素积累。此外，全基因组亚硫酸氢盐测序显示，接种后24小时甜瓜基因组DNA甲基化水平下降了6.15%。值得注意的是，CG位点表现出较高的甲基化水平和最大数量的差异甲基化区域（DMR）。此外，还鉴定出176个与防御反应相关的DMR相关基因（DMG）、134个活性氧代谢途径中的DMG和41个苯丙烷类代谢中的DMG。具有差异甲基化的差异表达基因主要受低甲基化影响，转录水平升高，参与苯丙烷类生物合成和次生代谢物生物合成。