Chu Chen, Li Dongxu, Gu Linqing, Yang Sihai, Liu Changhong
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
J Fungi (Basel). 2025 Apr 1;11(4):277. doi: 10.3390/jof11040277.
, a Basidiomycota fungus with a tetrapolar mating system, serves as a key model for studying sexual reproduction. In this study, two distinct mating subtypes (I and II) were identified in strain 20R-7-ZF01, isolated from subseafloor sediment, which exhibited eight different mating interaction phenotypes. Intra-subtypes exhibited colony-symmetric tetrapolar interactions (G1), whereas inter-subtype crosses yielded colony-asymmetric phenotypes (G2) and a reduced number of fruiting bodies. Nuclear migration analysis revealed that both subtypes follow the same sexual reproductive process, suggesting functional similarities despite the different reproductive outcomes. Gene silencing of mating-type loci identified the genes and within the B locus as key factors in determining mating subtype identity. Additionally, a similar pattern of mating subtype differentiation was observed in five other strains from both subseafloor and terrestrial environments. These findings highlight the genetic diversity within , challenge the classical understanding of fungal mating systems, and provide new insights into the genetic evolutionary mechanisms governing fungi with tetrapolar mating systems.
作为一种具有四极性交配系统的担子菌纲真菌，是研究有性生殖的关键模型。在本研究中，从海底沉积物中分离出的20R - 7 - ZF01菌株中鉴定出两种不同的交配亚型（I和II），该菌株表现出八种不同的交配相互作用表型。亚型内表现出菌落对称的四极性相互作用（G1），而亚型间杂交产生菌落不对称表型（G2）和子实体数量减少。核迁移分析表明，两种亚型遵循相同的有性生殖过程，这表明尽管生殖结果不同，但功能相似。交配型位点的基因沉默确定了B位点内的基因和为决定交配亚型身份的关键因素。此外，在来自海底和陆地环境的其他五种菌株中也观察到了类似的交配亚型分化模式。这些发现突出了内的遗传多样性，挑战了对真菌交配系统的传统理解，并为控制具有四极性交配系统的真菌的遗传进化机制提供了新的见解。
