Chu Chen, Li Dongxu, Liu Changhong
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
J Fungi (Basel). 2025 Jun 13;11(6):451. doi: 10.3390/jof11060451.
The A mating-type locus in , which encodes homeodomain (HD) transcription factors, is essential for regulating sexual compatibility and development. While the role of the Aα sublocus and its Y-Z HD protein complex is adequality understood, the function of HD proteins at the Aβ sublocus remains unclear. In this study, we analyzed the Aβ sublocus of eight monokaryotic strains derived from the parental dikaryotic strain 20R-7-Z01 and identified four HD genes, , , , and , located at the Aβ sublocus. These genes encode two HD1 proteins (S and Q) and two HD2 proteins (R and V). Protein structure prediction, interaction assays, and in vivo functional analyses revealed that R-S and V-Q interactions independently regulate sexual compatibility and fruiting body development. This research highlights the critical role of the Aβ sublocus in fungal reproduction and provides valuable insights for breeding edible and medicinal strains.
中的A交配型位点编码同源结构域（HD）转录因子，对调节性亲和性和发育至关重要。虽然对Aα亚位点及其Y-Z HD蛋白复合体的作用已有充分了解，但Aβ亚位点处HD蛋白的功能仍不清楚。在本研究中，我们分析了源自双亲双核菌株20R-7-Z01的八个单核菌株的Aβ亚位点，并鉴定出位于Aβ亚位点的四个HD基因，即、、、和。这些基因编码两种HD1蛋白（S和Q）和两种HD2蛋白（R和V）。蛋白质结构预测、相互作用分析和体内功能分析表明，R-S和V-Q相互作用独立调节性亲和性和子实体发育。本研究突出了Aβ亚位点在真菌繁殖中的关键作用，并为可食用和药用菌株的育种提供了有价值的见解。
