Paulo Carolina, Matos Joana, Afonso Cláudia, Cardoso Carlos
Division of Aquaculture and Upgrading (DivAV), Portuguese Institute for the Sea and Atmosphere (IPMA, IP), Avenida Alfredo Magalhães Ramalho, 6, 1495-006 Lisbon, Portugal.
Polytechnic Institute of Setúbal, Rua Américo da Silva Marinho, s/n, 2839-001 Lavradio, Portugal.
Mar Drugs. 2025 Mar 25;23(4):141. doi: 10.3390/md23040141.
The brown seaweed is a major invasive species in Europe, menacing local ecosystems. The challenge lies in assessing application routes for this biomass, testing different extraction technologies (overnight agitation, mechanical homogenization, pH-shift, ionic liquid-, and ultrasound-assisted extractions) and parameters. There was a higher yield in the extracts homogenized with 70% ethanol, especially with 1:20, /, biomass-solvent ratio, than in aqueous extracts. As to overnight agitation, 70% ethanol produced results (24.5-28.3%) similar to those found in the homogenized extracts. However, in the former, the best biomass-solvent proportion was 1:10, /. Mineral matter yield presented specific patterns, reaching 59.6 ± 1.1% (70% ethanol) and 82.3 ± 0.1% (water). The highest total polyphenol level was attained in the 70% ethanol, 1:20, /, extract, 310.7 ± 22.1 mg GAE/100 g dw seaweed. This extract had a higher FRAP/ABTS. The extract attained with overnight agitation, 70% ethanol, 1:10, /, had 48% COX-2 inhibition as anti-inflammatory activity. Besides showing the potential of for pharmacological purposes, especially in the antioxidant and anti-inflammatory area, this study enabled us to rank technologies and conditions for the utilization of this abundant biomass resource by the industry.
这种褐藻是欧洲的一种主要入侵物种，对当地生态系统构成威胁。挑战在于评估这种生物质的应用途径，测试不同的提取技术（过夜搅拌、机械匀浆、pH值变化、离子液体辅助和超声辅助提取）及参数。用70%乙醇匀浆得到的提取物产量更高，尤其是生物质与溶剂比例为1:20时，高于水提取物。至于过夜搅拌，70%乙醇得到的结果（24.5 - 28.3%）与匀浆提取物相似。然而，对于前者，最佳生物质与溶剂比例为1:10。矿物质产量呈现特定模式，70%乙醇提取时达到59.6 ± 1.1%，水提取时达到82.3 ± 0.1%。总多酚含量最高的是70%乙醇、1:20提取物，为310.7 ± 22.1 mg GAE/100 g干重海藻。该提取物具有更高的铁离子还原抗氧化能力（FRAP）/ABTS值。过夜搅拌、70%乙醇、1:10提取物具有48%的环氧合酶-2（COX-2）抑制率，作为抗炎活性。这项研究除了展示其在药理方面的潜力，特别是在抗氧化和抗炎领域，还使我们能够对该行业利用这种丰富生物质资源的技术和条件进行排序。
