CINBIO, Universidade de Vigo, Department of Chemical Engineering, Facultade de Ciencias, Campus Ourense, As Lagoas, 32004 Ourense, Spain.
Mar Drugs. 2023 May 25;21(6):319. doi: 10.3390/md21060319.
One possible scheme of biomass valorization based on a green, rapid and efficient fractionation technique was proposed. Microwave-assisted pressurized hot water extraction was the technology selected as the initial stage for the solubilization of different seaweed components. Operation at 180 °C for 10 min with a 30 liquid-to-solid ratio solubilized more than 40% of the initial material. Both the alginate recovery yield (3.2%) and the phenolic content of the water-soluble extracts (2.3%) were slightly higher when distilled water was used as solvent. However, the carbohydrate content in the extract (60%) was similar for both solvents, but the sulfate content was higher for samples processed with salt water collected from the same coast as the seaweeds. The antiradical capacity of the extracts was related to the phenolic content in the extracts, but the cytotoxicity towards HeLa229 cancer cells was highest (EC = 48 µg/mL) for the extract obtained with distilled water at the lowest temperature evaluated. Operation time showed a relevant enhancement of the extraction performance and bioactive properties of the soluble extracts. The further fractionation and study of this extract would be recommended to extend its potential applications. However, due to the low extraction yield, emphasis was given to the solid residue, which showed a heating value in the range 16,102-18,413 kJ/kg and could be useful for the preparation of biomaterials according to its rheological properties.
提出了一种基于绿色、快速、高效的生物质分馏技术的增值方案。选择微波辅助加压热水提取作为初始阶段，以溶解不同海藻成分。在 180°C 下操作 10 分钟，液固比为 30 时，超过 40%的初始材料被溶解。当使用蒸馏水作为溶剂时，海藻酸盐的回收率（3.2%）和水溶性提取物的酚含量（2.3%）略高。然而，两种溶剂的提取物中碳水化合物含量（60%）相似，但用来自与海藻同一海岸的盐水处理的样品中硫酸盐含量更高。提取物的抗氧化能力与提取物中的酚含量有关，但用蒸馏水在评估的最低温度下获得的提取物对 HeLa229 癌细胞的细胞毒性最高（EC=48µg/mL）。操作时间显著提高了可溶性提取物的提取性能和生物活性。建议进一步对该提取物进行分离和研究，以扩展其潜在应用。然而，由于提取率低，因此重点放在固体残渣上，其热值在 16,102-18,413kJ/kg 范围内，根据其流变性能可用于制备生物材料。
