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三种内生菌株对玉米的抑制作用研究

Study on the Inhibitory Effects of Three Endophytic Strains on in Maize.

作者信息

Ma Siyu, Li Min, Zhang Siqi, Yang Yin, Zhu Fengsha, Li Xingyu, Munir Shahzad, He Pengfei, He Pengbo, Wu Yixin, He Yueqiu, Tang Ping

机构信息

College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.

College of Science, Yunnan Agricultural University, Kunming 650201, China.

出版信息

Metabolites. 2025 Apr 11;15(4):268. doi: 10.3390/metabo15040268.

DOI:10.3390/metabo15040268
PMID:40278396
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12029785/
Abstract

BACKGROUND

Maize is easily contaminated by , and the aflatoxin produced by has been classified as a Group 1 carcinogen, for which there are currently no effective control measures. Biological control is regarded as an environmentally friendly and safe approach. Strains ZH179, ZH409, and ZH99 are three bacteria isolated from our laboratory that exhibit antagonistic effects against . We conducted experiments to investigate their biocontrol efficacy.

RESULTS

The experimental results demonstrated that these three strains effectively inhibited on plates and stored maize seeds. Identification revealed that ZH179 is , while ZH409 and ZH99 are . We also identified lipopeptide synthetase-related genes, including , , , , , , , , , and , in these three strains. Furthermore, LC-MS analysis confirmed that these strains could produce lipopeptide compounds such as surfactin, fengycin, iturin, and bacillomycin. Using the Oxford cup method, we found that the lipopeptide compounds produced by these strains can inhibit the growth of .

CONCLUSION

These findings suggest that strains ZH179, ZH409, and ZH99 have good control effects on during the storage of maize, primarily due to the lipopeptide compounds. This study provides a theoretical basis for using these three strains in the biological control of .

摘要

背景

玉米容易受到[具体污染源未提及]污染，[污染源]产生的黄曲霉毒素已被列为1类致癌物，目前尚无有效的控制措施。生物防治被认为是一种环境友好且安全的方法。菌株ZH179、ZH409和ZH99是从我们实验室分离出的三种对[具体抑制对象未提及]具有拮抗作用的细菌。我们进行了实验以研究它们的生物防治效果。

结果

实验结果表明，这三种菌株在平板和储存的玉米种子上均能有效抑制[具体抑制对象未提及]。鉴定显示ZH179是[具体菌种未提及]，而ZH409和ZH99是[具体菌种未提及]。我们还在这三种菌株中鉴定出了脂肽合成酶相关基因，包括[具体基因未提及]。此外，液相色谱 - 质谱分析证实这些菌株能够产生脂肽化合物，如表面活性素、丰原素、伊枯草菌素和芽孢杆菌霉素。使用牛津杯法，我们发现这些菌株产生的脂肽化合物能够抑制[具体抑制对象未提及]的生长。

结论

这些发现表明，菌株ZH179、ZH409和ZH99在玉米储存期间对[具体抑制对象未提及]具有良好的防治效果，主要是由于脂肽化合物。本研究为利用这三种菌株进行[具体防治对象未提及]的生物防治提供了理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e14/12029785/a3df14ab2bf1/metabolites-15-00268-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e14/12029785/bda918e30e6a/metabolites-15-00268-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e14/12029785/e4b559201655/metabolites-15-00268-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e14/12029785/ac9d35580b9a/metabolites-15-00268-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e14/12029785/a3df14ab2bf1/metabolites-15-00268-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e14/12029785/bda918e30e6a/metabolites-15-00268-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e14/12029785/e4b559201655/metabolites-15-00268-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e14/12029785/ac9d35580b9a/metabolites-15-00268-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e14/12029785/a3df14ab2bf1/metabolites-15-00268-g004.jpg

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Microb Ecol. 2024 Oct 28;87(1):133. doi: 10.1007/s00248-024-02445-5.
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Anti-Aflatoxigenic BC11-1 Exhibits Mycotoxin Detoxification, Phosphate Solubilization, and Cytokinin Production.抗黄曲霉毒素BC11-1具有霉菌毒素解毒、溶解磷酸盐和产生细胞分裂素的特性。
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