Kim Yu-Jin, Kim Hyeok, Lee Dong-Hyun, Kim Yeong Hwan, Park Jae-Hyun, Sim Woo-Sup, Kim Jin-Ju, Ban Kiwon, Um Soong Ho, Park Hyun Ji, Davis Michael E, Park Hun-Jun, Bhang Suk Ho
School of Chemical Engineering, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea.
Adv Sci (Weinh). 2025 Aug;12(29):e2500788. doi: 10.1002/advs.202500788. Epub 2025 Apr 25.
Despite the promising potential of stromal cell therapy in treating myocardial infarction (MI), its effectiveness is limited by poor cell retention and engraftment in ischemic environments. This study introduces a novel strategy that combines the preconditioning of human adipose-derived stromal cells (hADSCs) using OLED-based photobiomodulation (OPBM) and culturing these cells into 3D spheroids. The preconditioned 3D spheroids (APCS group) exhibit significantly enhanced angiogenic, arterialized, and tissue remodeling capabilities compared with those of traditional 2D cultures and non-preconditioned spheroids. In vivo transplantation of these spheroids into the border zone of infarcted area significantly improve cardiac function and reduce adverse remodeling by enhancing anti-fibrosis and angiogenesis including arterialization. The combined strategy with OPBM preconditioning and 3D spheroid culture system can enhance therapeutic potential of hADSCs with multiple paracrine effects for cardiac repair. This novel approach provides next generation of cell therapeutics to overcome the limitation of adult stromal cell therapy in patients with post-MI heart failure.
尽管基质细胞疗法在治疗心肌梗死（MI）方面具有广阔前景，但其有效性受到缺血环境中细胞保留和植入不佳的限制。本研究引入了一种新策略，即将基于有机发光二极管的光生物调节（OPBM）对人脂肪来源的基质细胞（hADSCs）进行预处理与将这些细胞培养成三维球体相结合。与传统二维培养和未预处理的球体相比，预处理后的三维球体（APCS组）表现出显著增强的血管生成、动脉化和组织重塑能力。将这些球体体内移植到梗死区域的边界区可通过增强抗纤维化和包括动脉化在内的血管生成，显著改善心脏功能并减少不良重塑。OPBM预处理和三维球体培养系统的联合策略可增强hADSCs的治疗潜力，其具有多种旁分泌作用促进心脏修复。这种新方法为克服成人基质细胞疗法在心肌梗死后心力衰竭患者中的局限性提供了新一代细胞治疗方法。
