Li Jie, Zhu Chengyong, Yang Chenbo, Zheng Quan, Wang Binhui, Tu Jingmin, Zhang Qian, Liu Sheng, Wang Xinfa, Qiao Jiangwei
Hubei Key Laboratory for High-efficiency Utilization of Solar Energy and Operation Control of Energy Storage System, Hubei University of Technology, Wuhan, 430068, China.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, China.
Plant Methods. 2025 Apr 25;21(1):54. doi: 10.1186/s13007-025-01373-w.
Rapeseed (Brassica napus L.) inflorescence coverage is a crucial phenotypic parameter for assessing crop growth and estimating yield. Accurate crop cover assessment is typically performed using Unmanned Aerial Vehicles (UAVs) in combination with semantic segmentation methods. However, the irregular and variable morphology of rapeseed inflorescences presents significant challenges in segmentation. To address these challenges, advanced methods that can improve segmentation accuracy, particularly under limited data conditions, are needed. In this study, we propose a cost-effective and high-throughput approach using a semi-supervised learning framework, DM_CorrMatch. This method enhances input images through strong and weak data augmentation techniques, while leveraging the Denoising Diffusion Probabilistic Model (DDPM) to generate additional samples in data-scarce scenarios. We propose an automatic update strategy for labeled data to dilute the proportion of erroneous labels in manual segmentation. Furthermore, a novel network architecture, Mamba-Deeplabv3+, is proposed, combining the strengths of Mamba and Convolutional Neural Networks (CNNs) for both global and local feature extraction. This architecture effectively captures key inflorescence features, even under varying poses, while reducing the influence of complex backgrounds. The proposed method is validated on the Rapeseed Flower Segmentation Dataset (RFSD), which consists of 720 UAV images from the Yangluo experimental station of the Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences (CAAS). The experimental results showed that our method outperforms four traditional segmentation methods and eleven deep learning methods, achieving an Intersection over Union (IoU) of 0.886, Precision of 0.942, and Recall of 0.940. The proposed semi-supervised learning-based method, combined with the Mamba-Deeplabv3+ architecture, demonstrates superior performance in accurately segmenting rapeseed inflorescences under challenging conditions. Our approach effectively handles complex backgrounds and various poses of inflorescences, providing a reliable tool for rapeseed flower cover estimation. This method can aid in the development of high-yield cultivars and improve crop monitoring through UAV-based technologies.
油菜(Brassica napus L.)花序覆盖率是评估作物生长和估算产量的关键表型参数。准确的作物覆盖评估通常使用无人机(UAV)结合语义分割方法来进行。然而,油菜花序不规则且多变的形态给分割带来了重大挑战。为应对这些挑战,需要能够提高分割精度的先进方法,特别是在数据有限的条件下。在本研究中,我们提出了一种使用半监督学习框架DM_CorrMatch的经济高效且高通量的方法。该方法通过强数据增强和弱数据增强技术增强输入图像,同时利用去噪扩散概率模型(DDPM)在数据稀缺的情况下生成额外样本。我们提出了一种标记数据的自动更新策略,以稀释手动分割中错误标签的比例。此外,还提出了一种新颖的网络架构Mamba-Deeplabv3+,它结合了Mamba和卷积神经网络(CNN)的优势,用于全局和局部特征提取。这种架构即使在姿态变化的情况下也能有效捕捉关键花序特征,同时减少复杂背景的影响。所提出的方法在油菜花花序分割数据集(RFSD)上得到验证,该数据集由中国农业科学院油料作物研究所阳逻实验站的720张无人机图像组成。实验结果表明,我们的方法优于四种传统分割方法和十一种深度学习方法,交并比(IoU)达到0.886,精度为0.942,召回率为0.940。所提出的基于半监督学习的方法与Mamba-Deeplabv3+架构相结合,在具有挑战性的条件下准确分割油菜花序方面表现出卓越性能。我们的方法有效地处理了复杂背景和花序的各种姿态,为油菜花花序覆盖估计提供了可靠工具。该方法有助于高产品种的开发,并通过基于无人机的技术改善作物监测。