Li Jifeng, Ruan Yong, Jiang Chuanmei, Sun Jinkui, An Dongwei, Zhou Bo, Liu Huan, Li Ziyang, Xu Houqiang
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China.
College of Animal Science, Guizhou University, Guiyang 550025, China.
Animals (Basel). 2025 Apr 10;15(8):1101. doi: 10.3390/ani15081101.
The gene, a member of the short-chain dehydrogenase/reductase (SDR) family, is involved in critical metabolic processes in animals. This study investigated the expression patterns of across various tissues of developmental stages in pigs and preliminarily evaluated its effects on myoblast proliferation, apoptosis, and differentiation. RT-qPCR (real-time quantitative PCR) was employed to analyze expression in the heart, liver, spleen, lungs, kidneys, longissimus dorsi, foreleg, and hind leg of pigs at 3 days, 6 months, and 12 months of age. Cell proliferation was analyzed using EdU (5-ethynyl-2'-deoxyuridine) assays, RT-qPCR, and flow cytometry, while the expression changes of proliferation-, apoptosis-, and differentiation-related genes were assessed via RT-qPCR. The results indicated that was expressed in all eight tissues at all three developmental stages. At 3 days, expression was the highest in the kidneys; at 6 months, it peaked in the liver; and at 12 months, it was again the highest in the kidneys. Across all stages, the liver and kidneys exhibited the highest expression levels. Functional studies revealed that overexpression suppressed myoblast proliferation and differentiation while promoting apoptosis. In contrast, inhibition enhanced myoblast proliferation and differentiation and reduced apoptosis. These findings underscore the regulatory role of in myogenesis and provide insights into its metabolic and developmental functions in pigs.
该基因是短链脱氢酶/还原酶（SDR）家族的成员之一，参与动物体内关键的代谢过程。本研究调查了该基因在猪不同发育阶段各组织中的表达模式，并初步评估了其对成肌细胞增殖、凋亡和分化的影响。采用RT-qPCR（实时定量PCR）分析该基因在3日龄、6月龄和12月龄猪的心脏、肝脏、脾脏、肺脏、肾脏、背最长肌、前腿和后腿中的表达情况。使用EdU（5-乙炔基-2'-脱氧尿苷）检测、RT-qPCR和流式细胞术分析细胞增殖情况，同时通过RT-qPCR评估增殖、凋亡和分化相关基因的表达变化。结果表明，该基因在所有三个发育阶段的所有八个组织中均有表达。在3日龄时，该基因在肾脏中的表达最高；在6月龄时，其在肝脏中达到峰值；在12月龄时，其在肾脏中再次最高。在所有阶段中，肝脏和肾脏中该基因的表达水平最高。功能研究表明，该基因的过表达抑制了成肌细胞的增殖和分化，同时促进了凋亡。相反，该基因的抑制增强了成肌细胞的增殖和分化，并减少了凋亡。这些发现强调了该基因在肌生成中的调节作用，并为其在猪体内的代谢和发育功能提供了见解。
