Hundam Seif, Al-Zghoul Mohammad Borhan, Ababneh Mustafa, Alanagreh Lo'ai, Dahadha Rahmeh, Mayyas Mohammad, Alghizzawi Daoud, Mustafa Minas A, Gerrard David E, Dalloul Rami A
Department of Basic Medical Veterinary Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan.
Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, The Hashemite University, Zarqa 13133, Jordan.
Animals (Basel). 2025 Apr 17;15(8):1149. doi: 10.3390/ani15081149.
Thermal manipulation (TM) during embryogenesis has emerged as a promising strategy to enhance post-hatch performance and improve resilience to environmental and bacterial stress, which offers a potential alternative to reduce the reliance on antibiotic growth promoters (AGPs) in broiler production. This study investigated TM's ability to modulate broilers' cecal microbiota and enhance resilience to lipopolysaccharide (LPS)-induced stress. Eggs in the control group (CON) were incubated at 37.8 °C and 56% relative humidity (RH), while TM eggs were exposed to 39 °C and 65% RH for 18 h daily from embryonic days 10-18. Post-hatch, the LPS subgroups (LPS-CON, LPS-TM) received intraperitoneal LPS injections, and body weight (BW) and temperature (BT) were monitored. Cecal samples were collected for microbiome sequencing. Alpha diversity showed no differences ( > 0.05), but beta diversity revealed differences between groups (PERMANOVA, < 0.05). and dominated the microbiota at the phylum level. were enriched in the TM groups ( < 0.001) and were increased in the LPS-CON group ( < 0.019). LPS reduced BT in the CON group ( < 0.01), but LPS-TM birds bypassed hypothermia. LPS significantly reduced BW ( < 0.001), while TM had no significant effect. These findings demonstrate TM's enduring influence on gut microbiota and stress resilience, highlighting its potential to reduce antibiotic reliance and mitigate antimicrobial resistance (AMR) in poultry production.
胚胎发育期间的热调控（TM）已成为一种有前景的策略，可提高孵化后性能，并增强对环境和细菌应激的恢复力，这为减少肉鸡生产中对抗生素生长促进剂（AGP）的依赖提供了一种潜在的替代方法。本研究调查了TM调节肉鸡盲肠微生物群以及增强对脂多糖（LPS）诱导应激的恢复力的能力。对照组（CON）的鸡蛋在37.8°C和56%相对湿度（RH）下孵化，而TM组的鸡蛋从胚胎第10天到第18天每天暴露于39°C和65%RH环境中18小时。孵化后，LPS亚组（LPS-CON、LPS-TM）接受腹腔注射LPS，并监测体重（BW）和体温（BT）。收集盲肠样本进行微生物组测序。α多样性无差异（>0.05），但β多样性显示组间存在差异（PERMANOVA，<0.05）。在门水平上， 和 主导微生物群。 在TM组中富集（<0.001），而 在LPS-CON组中增加（<0.019）。LPS降低了CON组的BT（<0.01），但LPS-TM组的鸡避免了体温过低。LPS显著降低了BW（<0.001），而TM没有显著影响。这些发现证明了TM对肠道微生物群和应激恢复力的持久影响，突出了其在减少家禽生产中抗生素依赖和减轻抗菌药物耐药性（AMR）方面的潜力。
