Nueraihemaiti Nuerbiye, Dilimulati Dilihuma, Baishan Alhar, Hailati Sendaer, Maihemuti Nulibiya, Aikebaier Alifeiye, Paerhati Yipaerguli, Zhou Wenting
Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China.
Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Urumqi 830017, China.
Biology (Basel). 2025 Apr 6;14(4):377. doi: 10.3390/biology14040377.
Extracellular vesicles (EVs) are those with a double-membrane structure that contains proteins, lipids, nucleic acids, and other biologically active substances that play an important role in cell-cell and cell-environment communication. They have also become an important mechanism for exchanging biologically active substances for cellular molecules. As many studies on EVs have been conducted, plant-derived extracellular vesicles (PDEVs) have also started attracting attention. The biological activity and stability of PDEVs are closely related to the extraction and separation methods, and choosing a separation method that meets the requirements of PDEVs is important. The extraction methods of PDEVs include ultracentrifugation, ultrafiltration, size-exclusion chromatography, etc. In recent years, it has been found through research that PDEVs possess biological properties, such as anti-inflammatory, anti-cancer, and anti-infective properties, and that they show unique advantages as therapeutic agents and drug carriers. Therefore, we have collected the scientific literature related to EVs derived from more than a dozen fruits and vegetables, and summarized and analyzed their extraction, separation, and roles in disease treatment, aiming to provide reference and inspiration for the in-depth study of the efficacy of new drugs.
细胞外囊泡（EVs）是具有双膜结构的囊泡，其包含蛋白质、脂质、核酸和其他生物活性物质，这些物质在细胞间和细胞与环境的通讯中发挥着重要作用。它们也已成为细胞分子交换生物活性物质的一种重要机制。随着对EVs的众多研究开展，植物源细胞外囊泡（PDEVs）也开始受到关注。PDEVs的生物活性和稳定性与提取和分离方法密切相关，选择符合PDEVs要求的分离方法很重要。PDEVs的提取方法包括超速离心、超滤、尺寸排阻色谱等。近年来，通过研究发现PDEVs具有抗炎、抗癌和抗感染等生物学特性，并且它们作为治疗剂和药物载体显示出独特优势。因此，我们收集了与十几种水果和蔬菜来源的EVs相关的科学文献，并对其提取、分离及其在疾病治疗中的作用进行了总结和分析，旨在为深入研究新药疗效提供参考和启示。
