[物种名称1]和[物种名称2]线粒体基因组的完整组装及比较分析

Assembly and Comparative Analysis of the Complete Mitochondrial Genomes of and .

作者信息

Liao Guojian, Liang Wenjing, Yu Haixia, Zhang Kun, Li Linxuan, Feng Shixin, Song Lisha, Yang Cuihong, Wan Lingyun, Zeng Dongqiang, Zhang Zhanjiang, Wei Shugen

机构信息

Guangxi Key Laboratory of High-Quality Formation and Utilization of Dao-Di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.

College of Agriculture, Guangxi University, Nanning 530024, China.

出版信息

Genes (Basel). 2025 Apr 14;16(4):450. doi: 10.3390/genes16040450.

BACKGROUND

() and (), two medicinally important species within the genus , have been widely used in Traditional Chinese Medicine (TCM) for the treatment of rheumatism, traumatic injuries, and related ailments. Despite their medicinal significance, research on the mitochondrial DNA (mtDNA) of species remains limited.

METHODS

We utilized NovaSeq 6000 and PromethION sequencing platforms to assemble the complete mitochondrial genomes of and , and conducted in-depth comparative genomic and evolutionary analyses.

RESULTS

The complete mitochondrial genomes of and were assembled and annotated, with total lengths of 535,215 bp and 471,049 bp, respectively. Both genomes encode 40 unique protein-coding genes (PCGs), composed of 24 core and 16 non-core genes, alongside multiple tRNA and rRNA genes. Repetitive element analysis identified 158 and 403 dispersed repeats in and , respectively, as well as 123 and 139 simple sequence repeats (SSRs). RNA editing site predictions revealed C-to-U conversions in both species. Additionally, chloroplast-to-mitochondrial DNA migration analysis detected 34 homologous fragments in and 28 homologous fragments in . Phylogenetically, . and cluster within the Liliales order and Smilacaceae family, closely related to Lilium species. Collinearity analysis indicated numerous syntenic blocks between Smilax and three other Liliopsida species, though gene order was not conserved.

CONCLUSIONS

This study presents high-quality mitochondrial genome assemblies for . and , providing valuable insights into molecular identification and conservation efforts of these traditional medicinal plants.

背景

菝葜属的两个具有重要药用价值的物种，（此处括号内容缺失），已在传统中药中广泛用于治疗风湿病、创伤性损伤及相关疾病。尽管它们具有药用意义，但对菝葜属物种线粒体DNA（mtDNA）的研究仍然有限。

方法

我们利用NovaSeq 6000和PromethION测序平台组装了（此处括号内容缺失）和（此处括号内容缺失）的完整线粒体基因组，并进行了深入的比较基因组和进化分析。

结果

（此处括号内容缺失）和（此处括号内容缺失）的完整线粒体基因组被组装并注释，总长度分别为535,215 bp和471,049 bp。两个基因组都编码40个独特的蛋白质编码基因（PCG），由24个核心基因和16个非核心基因组成，同时还有多个tRNA和rRNA基因。重复元件分析分别在（此处括号内容缺失）和（此处括号内容缺失）中鉴定出158个和403个分散重复序列，以及123个和139个简单序列重复（SSR）。RNA编辑位点预测显示两个物种中都存在C到U的转换。此外，叶绿体到线粒体DNA迁移分析在（此处括号内容缺失）中检测到34个同源片段，在（此处括号内容缺失）中检测到28个同源片段。在系统发育上，（此处括号内容缺失）和（此处括号内容缺失）聚类在百合目和菝葜科内，与百合属物种密切相关。共线性分析表明菝葜与其他三个单子叶植物物种之间存在许多同线区块，尽管基因顺序不保守。