从 L.中鉴定乙酸乙酯部分的成分并测定黄嘌呤氧化酶抑制活性。

Identification of the Constituents of Ethyl Acetate Fraction from L. and Determination of Xanthine Oxidase Inhibitory Properties.

机构信息

Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.

出版信息

Int J Mol Sci. 2023 Mar 8;24(6):5158. doi: 10.3390/ijms24065158.

The aim of this work was to investigate the xanthine oxidase (XO)-inhibitory activity of ethanol extracts from L. and to identify the active compounds in the ethyl acetate (EtOAc) fraction. Extraction of ethanol extracts from L. and then ethanol extracts were concentrated, and the polyphenolic compounds were extracted with petroleum ether (PE), chloroform, EtOAc, n-butanol (n-BuOH), and residual ethanol fractions. Their effects on XO activity were then compared separately. The polyphenolic components of the EtOAc fraction were identified by HPLC and HPLC-mass spectrometry (HPLC-MS) analysis. Kinetic analysis demonstrated that all these extracts showed XO-inhibitory properties, and among them the EtOAc fraction had the strongest inhibitory effect (IC = 101.04 μg/mL). The inhibitory constant () of the EtOAc fraction on XO activity was 65.20 μg/mL, showing excellent inhibition on XO in the competitive mode. Sixteen compounds were identified from the EtOAc fraction. The study demonstrates that the EtOAc fraction of L. may be a potential functional food to inhibit XO activity.

本研究旨在探讨从 L. 中提取的乙醇提取物对黄嘌呤氧化酶（XO）的抑制活性，并鉴定乙酸乙酯（EtOAc）馏分中的活性化合物。从 L. 中提取乙醇提取物，然后浓缩乙醇提取物，用石油醚（PE）、氯仿、乙酸乙酯、正丁醇（n-BuOH）和残余乙醇级分提取多酚化合物。然后分别比较它们对 XO 活性的影响。用高效液相色谱法（HPLC）和高效液相色谱-质谱联用（HPLC-MS）分析鉴定 EtOAc 馏分中的多酚成分。动力学分析表明，所有这些提取物均表现出 XO 抑制特性，其中 EtOAc 馏分的抑制作用最强（IC = 101.04 μg/mL）。EtOAc 馏分对 XO 活性的抑制常数（）为 65.20 μg/mL，以竞争性模式对 XO 表现出优异的抑制作用。从 EtOAc 馏分中鉴定出 16 种化合物。该研究表明， L. 的 EtOAc 馏分可能是一种具有抑制 XO 活性的潜在功能性食品。