Dalkılıç Semih, Kadıoğlu Dalkılıç Lütfiye, İsbenov Elgun, Uygur Lütfü, Taşdemir Ceydanur
Department of Biology and Molecular Biology and Genetics Program, Faculty of Science, Fırat University, 23119 Elazığ, Türkiye.
Life (Basel). 2025 Apr 16;15(4):651. doi: 10.3390/life15040651.
Cancer is a common disease worldwide, and medicinal plants are widely used for its treatment. is a plant from the Thymelaeaceae family that is endemic to East Asia. Popularly known as the agar tree, this plant is used in the treatment of heart diseases, asthma, cough, ulcers, gout, inflammation, and pain. In this study, the cytotoxic, apoptotic/necrotic, and antioxidant activities of root extracts of and their effects on gene expression were investigated. Cytotoxic effects were analyzed by the MTT assay method, apoptotic/necrotic effects by the double staining method, and antioxidant effects by the DPPH radical scavenging assay. According to the results, the best cytotoxic effect in HepG2 cells was observed at 1000 µg/mL hexane extract. In MCF-7 cells, 250 µg/mL hexane extract showed the best activity. Antioxidant activity was highest in dH2O and lowest in the methanol extract. In gene expression analysis, hexane and methanol extracts decreased p53 expression in HepG2 cells, while acetone extract increased it. In addition, the expression of Bax and Bcl-2 genes generally increased. This study revealed the potent cytotoxic, antioxidant, and apoptotic effects of the extract.
癌症是一种全球常见的疾病，药用植物被广泛用于其治疗。[植物名称]是一种来自瑞香科的植物，原产于东亚。这种植物俗称沉香树，用于治疗心脏病、哮喘、咳嗽、溃疡、痛风、炎症和疼痛。在本研究中，对[植物名称]根提取物的细胞毒性、凋亡/坏死和抗氧化活性及其对基因表达的影响进行了研究。细胞毒性作用通过MTT法分析，凋亡/坏死作用通过双重染色法分析，抗氧化作用通过DPPH自由基清除试验分析。结果显示，在HepG2细胞中，1000µg/mL己烷提取物的细胞毒性作用最佳。在MCF-7细胞中，250µg/mL己烷提取物表现出最佳活性。抗氧化活性在蒸馏水提取物中最高，在甲醇提取物中最低。在基因表达分析中，己烷和甲醇提取物降低了HepG2细胞中p53的表达，而丙酮提取物则增加了其表达。此外，Bax和Bcl-2基因的表达总体上有所增加。本研究揭示了[植物名称]提取物具有强大的细胞毒性、抗氧化和凋亡作用。
