Alam Janey, Mujahid Md, Jahan Yasmeen, Bagga Paramdeep, Rahman Md Azizur
Bioactive Research Laboratory, Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh 226026, India.
J Tradit Complement Med. 2016 Feb 3;7(1):9-13. doi: 10.1016/j.jtcme.2015.12.006. eCollection 2017 Jan.
Many traditional systems of medicines employ herbal drugs for the hepatoprotection. Aim of the study was designed to evaluate the hepatoprotective potential of 'ethanolic extract of ( Chen Xiang) leaves' (AAE) against paracetamol (PCM) induced hepatotoxicity in SD rats. Group I animals were treated with 1% CMC for 8 days. Group II, III, IV and V animals were first treated with '1% CMC' 1 ml/kg/day, AAE 200 mg/kg/day, AAE 400 mg/kg/day and silymarin 100 mg/kg/day respectively for 7 days and then, orally administered with PCM 3 g/kg b. wt. on 8th day in a single dose. 24 h after the last dosing by PCM, the blood was obtained through the retro-orbital plexus under light anesthesia and the animals were sacrificed. Hepatoprotective potential was assessed by various biochemical parameters such as ALT, AST, ALP, LDH, bilirubin, cholesterol, TP and ALB. Group IV rats showed significant ( < 0.01) decrease in ALT, AST, ALP, LDH, cholesterol, bilirubin, liver wt. and relative liver wt. levels while significant ( < 0.01) increase in final b. wt., TP and ALB levels as compared to group II rats. Hepatoprotective potential of AAE 400 mg/kg/day was comparable to that of standard drug silymarin 100 mg/kg/day. Results of the study were well supported by the histopathological observations. This study confirms that AAE possesses hepatoprotective potential comparable to that of standard drug silymarin as it exhibited comparable protective potential against PCM induced hepatotoxicity in SD rats.
许多传统医学体系都使用草药来保护肝脏。本研究旨在评估“沉香叶乙醇提取物”（AAE）对扑热息痛（PCM）诱导的SD大鼠肝毒性的肝脏保护潜力。第一组动物用1%羧甲基纤维素（CMC）处理8天。第二组、第三组、第四组和第五组动物首先分别用“1% CMC”1毫升/千克/天、AAE 200毫克/千克/天、AAE 400毫克/千克/天和水飞蓟宾100毫克/千克/天处理7天，然后在第8天口服给予PCM 3克/千克体重，单次给药。在PCM最后一次给药24小时后，在轻度麻醉下通过眶后丛取血，并处死动物。通过各种生化参数如谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）、胆红素、胆固醇、总蛋白（TP）和白蛋白（ALB）来评估肝脏保护潜力。与第二组大鼠相比，第四组大鼠的ALT、AST、ALP、LDH、胆固醇、胆红素、肝脏重量和相对肝脏重量水平显著（P<0.01）降低，而最终体重、TP和ALB水平显著（P<0.01）升高。AAE 400毫克/千克/天的肝脏保护潜力与标准药物水飞蓟宾100毫克/千克/天相当。组织病理学观察结果很好地支持了该研究结果。本研究证实，AAE具有与标准药物水飞蓟宾相当的肝脏保护潜力，因为它在SD大鼠中对PCM诱导的肝毒性表现出相当的保护潜力。
