Xu Guohao, Cai Gaozhe, Liang Lijuan, Cheng Jianxin, Song Lujie, Sun Rui, Shen Feng, Liu Bo, Feng Shilun, Zhang Jin
College of Life and Geographic Sciences, Kashi University, Kashi 844000, China.
Jiaxing Key Laboratory of Biosemiconductors (A), Xiangfu Laboratory, Jiashan 314102, China.
Micromachines (Basel). 2025 Apr 14;16(4):462. doi: 10.3390/mi16040462.
Waterborne pathogens seriously threaten human life and can cause diarrhea, gastrointestinal disorders, and more serious systemic infections. These pathogens are usually caused by contaminated water sources that contain disease-causing microorganisms, such as bacteria, viruses, and parasites, which cause infection and disease when they enter the human body through drinking water or other means. Due to the wide range of transmission routes and the high potential risk of waterborne pathogens, there is an urgent need for an ultrasensitive, rapid, and specific pathogenic microorganism monitoring platform to meet the critical monitoring needs of some water bodies' collection points daily monitoring needs. Microfluidics-based pathogen surveillance methods are an important stage towards automated detection through real-time and multi-targeted monitoring, thus enabling a comprehensive assessment of the risk of exposure to waterborne pathogens and even emerging microbial contaminants, and thus better protection of public health. Therefore, this paper reviews the latest research results on the isolation and detection of waterborne pathogens based on microfluidic methods. First, we introduce the traditional methods for isolation and detection of pathogens. Then, we compare some existing microfluidic pathogen isolation and detection methods and finally look forward to some future research directions and applications of microfluidic technology in waterborne pathogens monitoring.
水传播病原体严重威胁人类生命，可导致腹泻、胃肠道疾病以及更严重的全身感染。这些病原体通常由受污染的水源引起，这些水源含有致病微生物，如细菌、病毒和寄生虫，它们通过饮用水或其他途径进入人体后会引发感染和疾病。由于水传播病原体的传播途径广泛且潜在风险高，迫切需要一个超灵敏、快速且特异的致病微生物监测平台，以满足一些水体采集点日常监测的关键需求。基于微流控的病原体监测方法是通过实时和多靶点监测实现自动化检测的重要阶段，从而能够全面评估接触水传播病原体甚至新出现的微生物污染物的风险，进而更好地保护公众健康。因此，本文综述了基于微流控方法的水传播病原体分离与检测的最新研究成果。首先，我们介绍病原体分离与检测的传统方法。然后，我们比较一些现有的微流控病原体分离与检测方法，最后展望微流控技术在水传播病原体监测方面未来的一些研究方向和应用。
