Department of Basic Science & Humanities, SVKM'S NMIMS Mukesh Patel School of Technology Management & Engineering, Mumbai, Maharashtra 400056, India.
Department of Biotechnology Engineering, Kolhapur Institute of Technology's College of Engineering, Kolhapur 416 234, India.
Int J Biol Macromol. 2023 Dec 31;253(Pt 6):127358. doi: 10.1016/j.ijbiomac.2023.127358. Epub 2023 Oct 10.
Conventional techniques for enzyme immobilization suffer from suboptimal activity recovery due to insufficient enzyme loading and inadequate stability. Furthermore, these techniques are time-consuming and involve multiple steps which limit the applicability of immobilized enzymes. In contrast, the use of microfluidic devices for enzyme immobilization has garnered significant attention due to its ability to precisely control immobilization parameters, resulting in highly active immobilized enzymes. This approach offers several advantages, including reduced time and energy consumption, enhanced mass-heat transfer, and improved control over the mixing process. It maintains the superior structural configuration in immobilized form which ultimately affects the overall efficiency. The present review article comprehensively explains the design, construction, and various methods employed for enzyme immobilization using microfluidic devices. The immobilized enzymes prepared using these techniques demonstrated excellent catalytic activity, remarkable stability, and outstanding recyclability. Moreover, they have found applications in diverse areas such as biosensors, biotransformation, and bioremediation. The review article also discusses potential future developments and foresees significant challenges associated with enzyme immobilization using microfluidics, along with potential remedies. The development of this advanced technology not only paves the way for novel and innovative approaches to enzyme immobilization but also allows for the straightforward scalability of microfluidic-based techniques from an industrial standpoint.
传统的酶固定化技术由于酶负载量不足和稳定性不足，导致酶活性恢复不理想。此外，这些技术耗时且涉及多个步骤，限制了固定化酶的适用性。相比之下，微流控设备用于酶固定化由于能够精确控制固定化参数，从而获得高活性的固定化酶，因此受到了广泛关注。这种方法具有减少时间和能源消耗、增强质量传递、改善混合过程控制等优点。它以固定化形式保持了优越的结构配置，这最终影响了整体效率。本文综述了使用微流控设备进行酶固定化的设计、构建和各种方法。使用这些技术制备的固定化酶表现出优异的催化活性、显著的稳定性和出色的可回收性。此外，它们已在生物传感器、生物转化和生物修复等多个领域得到应用。本文还讨论了使用微流控技术进行酶固定化的潜在未来发展和预期挑战，以及潜在的解决方案。这项先进技术的发展不仅为酶固定化开辟了新的创新途径，而且从工业角度来看，还可以实现基于微流控的技术的直接扩展。
