Bioconversion of FeO Nanoparticles by Probiotics.

: Iron deficiency anemia remains a primary global health concern, affecting millions worldwide. Despite the widespread availability of iron supplements, their efficacy is often hindered by poor bioavailability and adverse gastrointestinal effects. This study explores the potential of probiotics to enhance the bioavailability of FeO NPs through probiotic-mediated mechanisms. : , , and were utilized to investigate their interactions with FeO NPs, synthesized via co-precipitation and characterized using transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. : The results indicated that probiotics adhere to the nanoparticle surface, with exhibiting the highest adhesion and internalization capacity, leading to a significant increase in 4-hydroxyphenylacetic acid (4-HPLA) production (11.73 ± 0.09 mg/mL at 24 h, < 0.05). Spectroscopic analyses further revealed that probiotic metabolism facilitates the oxidation of FeO to FeO. Additionally, FeO nanoparticle-treated cultures demonstrated enhanced bacterial viability and metabolic activity, highlighting a synergistic effect between probiotics and iron nanoparticles. : These findings provide compelling evidence for probiotic-assisted iron supplementation as a promising strategy to enhance iron bioavailability while mitigating the gastrointestinal side effects of conventional iron supplements.