Sun Yaqi, Li Sudan, Wu Xiang, Zhu Jiao, Dong Fei, Pei Zhaoshun, Li Zhenguo, Zhao Shanxing, Wang Chengpeng
Shandong Engineering Research Center of Ecological Horticultural Plant Breeding, Institute of Leisure Agriculture, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Shandong Haoyu Horticulture Technology Limited Liability Company, Rizhao 276800, China.
Plants (Basel). 2025 Apr 8;14(8):1155. doi: 10.3390/plants14081155.
Heat stress transcription factors (Hsfs) are crucial transcription factors (TFs) in plants, playing pivotal roles in responding to abiotic stresses. However, their specific functions in regulating heat stress responses in roses are not yet fully elucidated. Here, we cloned an Hsf gene, , from the rose variety 'Samantha'. This gene contains a coding sequence (CDS) of 1086 bp, encoding 361 amino acids. The RhHsfA7 protein has a molecular weight of 41.21 kDa, an isoelectric point of 5.41, and no signal peptide or transmembrane structure. Phylogenetic analyses revealed that is most closely related to , , and in , and is phylogenetically closer to species compared to other species. The RhHsfA7 protein possesses conserved domains, including an oligomerization domain (OD), a nuclear localization signal (NLS), a DNA-binding domain (DBD), and a nuclear export signal (NES), as well as the HsfA subfamily-specific transcriptional activation domain (AHA). RhHsfA7 was localized in the nucleus and exhibited transcriptional activation activity. Expression analysis revealed that was highly expressed in roots and leaves, and its expression was heat-specific. In rose leaves, through silencing and transient overexpression experiments, we discovered that silencing resulted in heat sensitivity, whereas transient overexpression of increased heat tolerance. Collectively, our findings suggest that positively regulates tolerance to heat stress in roses.
热胁迫转录因子（Hsfs）是植物中至关重要的转录因子（TFs），在应对非生物胁迫中发挥着关键作用。然而，它们在调控玫瑰热胁迫反应中的具体功能尚未完全阐明。在此，我们从玫瑰品种‘萨曼莎’中克隆了一个Hsfs基因， 。该基因包含一个1086 bp的编码序列（CDS），编码361个氨基酸。RhHsfA7蛋白的分子量为41.21 kDa，等电点为5.41，且无信号肽或跨膜结构。系统发育分析表明， 与 中的 、 和 关系最为密切，与其他物种相比，在系统发育上更接近 物种。RhHsfA7蛋白具有保守结构域，包括一个寡聚化结构域（OD）、一个核定位信号（NLS）、一个DNA结合结构域（DBD）和一个核输出信号（NES），以及HsfA亚家族特异性转录激活结构域（AHA）。RhHsfA7定位于细胞核并表现出转录激活活性。表达分析表明， 在根和叶中高度表达，且其表达具有热特异性。在玫瑰叶片中，通过沉默和瞬时过表达实验，我们发现沉默 导致热敏感性，而 的瞬时过表达提高了耐热性。总体而言，我们的研究结果表明， 正向调控玫瑰对热胁迫的耐受性。
