Shen Jiamin, Ding Kexin, Yu Zhiming, Zhang Yuzhen, Ni Jun, Wu Yuhuan
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 311121, China.
Plants (Basel). 2025 Apr 10;14(8):1178. doi: 10.3390/plants14081178.
Calcium ions (Ca) are an important secondary messenger in plant signal transduction networks. The cytosolic free Ca concentration ([Ca]) of plants changes rapidly when they are subjected to different abiotic stresses, which drives calcium signaling. Although this process has been extensively studied in spermatophytes, the details of calcium signaling in bryophytes remains largely unknown. In our study, we reconstituted aequorin in the bryophyte , optimized the percentage of ethanol in the Ca discharging solution, and measured the [Ca] changes induced by different stresses. In addition, we observed that the sources of Ca accessed following exposure to cold, drought, salt, and oxidative stress were different. Furthermore, we showed that long-term saline environments could suppress the basal [Ca] of , and the peak value of [Ca] induced by different stresses was lower than that of plants growing in non-stressed environments. This is the first systematic study of calcium signaling in bryophytes, and we provided an efficient and convenient tool to study calcium signaling in response to different abiotic stresses in bryophytes.
钙离子（Ca）是植物信号转导网络中一种重要的第二信使。当植物受到不同的非生物胁迫时，其胞质游离钙浓度（[Ca]）会迅速变化，从而驱动钙信号传导。尽管这一过程在种子植物中已得到广泛研究，但苔藓植物中钙信号传导的细节仍 largely 未知。在我们的研究中，我们在苔藓植物中重组了水母发光蛋白，优化了钙释放溶液中的乙醇百分比，并测量了不同胁迫诱导的[Ca]变化。此外，我们观察到暴露于寒冷、干旱、盐和氧化胁迫后获取钙的来源不同。此外，我们表明长期盐环境会抑制苔藓植物的基础[Ca]，并且不同胁迫诱导的[Ca]峰值低于在非胁迫环境中生长的植物。这是对苔藓植物钙信号传导的首次系统研究，我们提供了一种高效便捷的工具来研究苔藓植物对不同非生物胁迫的钙信号传导响应。
