Belobrajdic Damien P, Dallimore Julie A, Adams Michael J, Singh Surinder P, Abeywardena Mahinda Y
CSIRO Health and Biosecurity, Adelaide, SA 5000, Australia.
Alliance for Research in Exercise, Nutrition and Activity (ARENA), University of South Australia, Adelaide, SA 5001, Australia.
Nutrients. 2025 Apr 9;17(8):1306. doi: 10.3390/nu17081306.
BACKGROUND/OBJECTIVES: Regiospecificity in triacylglycerols (TAGs) influences absorption/bioavailability of dietary fatty acids. We evaluated whether sn1,3 located DHA (22:6ω3) of a transgenic higher plant (DHA-Canola) preferentially facilitates its tissue incorporation as compared to sn2 positioned DHA (DHASCO of algal origin).
Sprague Dawley rats were fed diets (12 weeks) containing DHA-Canola or DHA-Control (a blend of DHASCO and high oleic sunflower seed oil (HOSO)) at 0.3%, 1%, 3%, and 6% (/), or 7% HOSO prior to determination of tissue fatty acids.
At 0.3 and 1% / supplementation, plasma, liver and cardiac tissue DHA incorporation was higher in the plant-based oil (DHA-Canola vs. DHA-Control; < 0.05), whilst sn2 enriched algal oil yielded better outcomes at higher doses (at 3% inclusion, plasma values were 7.8 vs. 5.9%, and at 6% supplementation, 10.0 vs. 7.9 in favor of DHA-Control, < 0.05) At lower intakes, sn1,3 regiospecificity (DHA-Canola) increased the omega-3 index, a clinically relevant biomarker, compared to DHA-Control ( < 0.05). Similarly, a build-up of 20:5ω3 and 22:5ω3 occurred with DHA-Canola. Consequently, total omega3s were higher in this latter group.
At lower intakes, sn1,3 regiospecificity of DHA leads to its preferential tissue incorporation compared to sn2 DHA.
背景/目的：三酰甘油（TAGs）中的区域特异性会影响膳食脂肪酸的吸收/生物利用度。我们评估了转基因高等植物（DHA-油菜）中位于sn1,3位的DHA（22:6ω3）与位于sn2位的DHA（藻类来源的DHASCO）相比，是否更有利于其在组织中的掺入。
在测定组织脂肪酸之前，给Sprague Dawley大鼠喂食含0.3%、1%、3%和6%（/）的DHA-油菜或DHA-对照（DHASCO和高油酸向日葵籽油（HOSO）的混合物）或7% HOSO的饲料12周。
在0.3%和1% /的添加量下，植物性油（DHA-油菜与DHA-对照相比；<0.05）中血浆、肝脏和心脏组织中的DHA掺入量更高，而富含sn2的藻油在较高剂量时效果更好（在3%的添加量下，血浆值分别为7.8%和5.9%，在6%的添加量下，分别为10.0%和7.9%，DHA-对照更有利，<0.05）。在较低摄入量时，与DHA-对照相比，sn1,3区域特异性（DHA-油菜）增加了ω-3指数，这是一个临床相关的生物标志物（<0.05）。同样，DHA-油菜中20:5ω3和22:5ω3也有所积累。因此，后一组中的总ω-3含量更高。
在较低摄入量时，与sn2 DHA相比，DHA的sn1,3区域特异性导致其在组织中更优先掺入。
