植物源（低芥酸菜籽来源）DHA（二十二碳六烯酸，22:6ω-3）的Sn1,3区域特异性使其在低膳食添加水平下，相比于藻油中的Sn2 DHA，能更优先地整合到大鼠组织中。

Sn1,3 Regiospecificity of DHA (22:6ω-3) of Plant Origin (DHA-Canola) Facilitates Its Preferential Tissue Incorporation in Rats Compared to sn2 DHA in Algal Oil at Low Dietary Inclusion Levels.

作者信息

Belobrajdic Damien P, Dallimore Julie A, Adams Michael J, Singh Surinder P, Abeywardena Mahinda Y

机构信息

CSIRO Health and Biosecurity, Adelaide, SA 5000, Australia.

Alliance for Research in Exercise, Nutrition and Activity (ARENA), University of South Australia, Adelaide, SA 5001, Australia.

出版信息

Nutrients. 2025 Apr 9;17(8):1306. doi: 10.3390/nu17081306.

DOI:10.3390/nu17081306

PMID:40284170

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12030648/

Abstract

BACKGROUND/OBJECTIVES: Regiospecificity in triacylglycerols (TAGs) influences absorption/bioavailability of dietary fatty acids. We evaluated whether sn1,3 located DHA (22:6ω3) of a transgenic higher plant (DHA-Canola) preferentially facilitates its tissue incorporation as compared to sn2 positioned DHA (DHASCO of algal origin).

METHODS

Sprague Dawley rats were fed diets (12 weeks) containing DHA-Canola or DHA-Control (a blend of DHASCO and high oleic sunflower seed oil (HOSO)) at 0.3%, 1%, 3%, and 6% (/), or 7% HOSO prior to determination of tissue fatty acids.

RESULTS

At 0.3 and 1% / supplementation, plasma, liver and cardiac tissue DHA incorporation was higher in the plant-based oil (DHA-Canola vs. DHA-Control; < 0.05), whilst sn2 enriched algal oil yielded better outcomes at higher doses (at 3% inclusion, plasma values were 7.8 vs. 5.9%, and at 6% supplementation, 10.0 vs. 7.9 in favor of DHA-Control, < 0.05) At lower intakes, sn1,3 regiospecificity (DHA-Canola) increased the omega-3 index, a clinically relevant biomarker, compared to DHA-Control ( < 0.05). Similarly, a build-up of 20:5ω3 and 22:5ω3 occurred with DHA-Canola. Consequently, total omega3s were higher in this latter group.

CONCLUSIONS

At lower intakes, sn1,3 regiospecificity of DHA leads to its preferential tissue incorporation compared to sn2 DHA.

摘要

背景/目的：三酰甘油（TAGs）中的区域特异性会影响膳食脂肪酸的吸收/生物利用度。我们评估了转基因高等植物（DHA-油菜）中位于sn1,3位的DHA（22:6ω3）与位于sn2位的DHA（藻类来源的DHASCO）相比，是否更有利于其在组织中的掺入。

方法

在测定组织脂肪酸之前，给Sprague Dawley大鼠喂食含0.3%、1%、3%和6%（/）的DHA-油菜或DHA-对照（DHASCO和高油酸向日葵籽油（HOSO）的混合物）或7% HOSO的饲料12周。

结果

在0.3%和1% /的添加量下，植物性油（DHA-油菜与DHA-对照相比；<0.05）中血浆、肝脏和心脏组织中的DHA掺入量更高，而富含sn2的藻油在较高剂量时效果更好（在3%的添加量下，血浆值分别为7.8%和5.9%，在6%的添加量下，分别为10.0%和7.9%，DHA-对照更有利，<0.05）。在较低摄入量时，与DHA-对照相比，sn1,3区域特异性（DHA-油菜）增加了ω-3指数，这是一个临床相关的生物标志物（<0.05）。同样，DHA-油菜中20:5ω3和22:5ω3也有所积累。因此，后一组中的总ω-3含量更高。