Ćorović Marija, Petrov Ivanković Anja, Milivojević Ana, Pfeffer Klaus, Homey Bernhard, Jansen Patrick A M, Zeeuwen Patrick L J M, van den Bogaard Ellen H, Bezbradica Dejan
Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Innovation Center, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, Serbia.
Pharmaceutics. 2025 Apr 8;17(4):487. doi: 10.3390/pharmaceutics17040487.
: Numerous intrinsic and extrinsic stressors can disrupt the balance of the skin microbiome, leading to the development of various skin diseases. It has been proven that coagulase-negative staphylococci (CoNS) are important commensals for maintaining skin microbiome homeostasis and fighting cutaneous pathogens such as (). Here, we examined the influence of polyphenol-rich enzymatic blackcurrant extract (EBCE) on pathogenic coagulase-positive strains and beneficial CoNS, like (), to explore its potential for rebalancing the skin microbiota. : The polyphenol profile of EBCE was determined by ultra-high-pressure liquid chromatography-tandem mass spectrometry. Microwell plate assays were employed to study the effect of EBCE on five strains isolated from the skin of atopic dermatitis patients. An in vitro human model was used to test its effect on mixed bacterial cultures. : EBCE inhibited the growth of all tested strains by 80-100% at the highest tested concentration after 7 h. No microbial growth was observed at the highest tested EBCE concentration using the model inoculated with one selected pathogen ( SA-DUS-017) and one commensal laboratory strain ( DSM 20044). The lowest tested concentration did not interfere with growth but strongly stimulated the growth of (~300-fold colony forming unit increase). In addition, low EBCE concentrations strongly stimulated CoNS growth in microbiome samples taken from the armpits of healthy volunteers that were spiked with SA-DUS-017. : These preclinical data support further testing of EBCE-enriched topical preparations as potential cutaneous prebiotics in human studies.
许多内在和外在应激源会破坏皮肤微生物群的平衡，导致各种皮肤疾病的发生。已证实凝固酶阴性葡萄球菌（CoNS）是维持皮肤微生物群稳态和对抗皮肤病原体（如……）的重要共生菌。在此，我们研究了富含多酚的酶解黑加仑提取物（EBCE）对致病性凝固酶阳性……菌株和有益的CoNS（如……）的影响，以探索其重新平衡皮肤微生物群的潜力。
通过超高压液相色谱 - 串联质谱法测定EBCE的多酚谱。采用微孔板试验研究EBCE对从特应性皮炎患者皮肤分离的5株……菌株的作用。使用体外人体……模型测试其对混合细菌培养物的影响。
在7小时后，在最高测试浓度下，EBCE对所有测试的……菌株的生长抑制率为80 - 100%。使用接种了一种选定病原体（SA - DUS - 017）和一种共生实验室菌株（DSM 20044）的……模型，在最高测试EBCE浓度下未观察到微生物生长。最低测试浓度不干扰……生长，但强烈刺激……的生长（菌落形成单位增加约300倍）。此外，低EBCE浓度强烈刺激了从接种了SA - DUS - 017的健康志愿者腋窝采集的微生物群样本中CoNS的生长。
这些临床前数据支持在人体研究中进一步测试富含EBCE的局部制剂作为潜在的皮肤益生元。
