Curcumin Microcapsule Formulations for Prolong Persistence in the Photodynamic Inactivation of Larvae.

Viral diseases including dengue, zika, chikungunya, and yellow fever remain a significant public health challenge, primarily due to the increasing resistance of these vectors, the mosquito, to conventional control methods. Herein, a microencapsulated curcumin formulation was developed and characterized using spray-drying technology, with D-mannitol and starch as encapsulating agents. After microencapsulation, photolarvicidal tablet formulations (Formulated Curcumin Tablets-FCT) were prepared, varying the proportions of starch and pectin: FCT1 (60% starch), FCT2 (35% pectin and 25% starch), and FCT3 (42.5% pectin and 17.5% starch), while maintaining 10% curcumin and 30% D-mannitol in all formulations. The main goal was to enhance the stability and efficacy of curcumin as a photolarvicidal agent. The formulation was characterized by UV-Vis spectroscopy, confocal microscopy, thermal analysis (TG and DSC), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR), and photodegradation assays under fluorescent light. The photodynamic inactivation (PDI) of larvae was evaluated under white, fluorescent light exposure, and the formulation exhibited a significantly enhanced larvicidal activity compared to free curcumin, with a 57-fold reduction in LC (LC = 0.27 mg/L). Additionally, the most effective formulation, FCT2, maintained its residual activity for 27 days, reinforcing that curcumin microencapsulation, combined with PDI, can extend vector control. Release studies under different pH conditions confirmed a controlled release mechanism, favoring environmental stability. The results indicate that microencapsulated curcumin has great potential as a sustainable photoinsecticidal agent, offering stability, efficacy, and a promising alternative for managing larval populations.