de Souza Larissa Marila, Venturini Francine Perri, Inada Natalia Mayumi, Iermak Ievgeniia, Garbuio Matheus, Mezzacappo Natasha Ferreira, de Oliveira Kleber Thiago, Bagnato Vanderlei Salvador
Federal University of São Carlos, PPG Biotec, São Carlos 13565-905, São Paulo, Brazil; São Carlos Institute of Physics, University of São Paulo, São Carlos 13560-970, São Paulo, Brazil.
São Carlos Institute of Physics, University of São Paulo, São Carlos 13560-970, São Paulo, Brazil.
Photodiagnosis Photodyn Ther. 2020 Sep;31:101840. doi: 10.1016/j.pdpdt.2020.101840. Epub 2020 May 30.
Combating the Aedes aegypti vector is still the key to control the transmission of many arboviruses, such as Dengue, Zika, and Chikungunya. As few products are efficient for Aedes aegypti control, the search for new strategies have become pivotal., t Substances with photodynamic activity, such as curcumin and their formulations, are strongly encouraged, due to their multi-target mechanism of action. In this study, we evaluated the photolarvicidal and ovicidal activity of curcumin in the presence of sucrose (named SC) and d-mannitol (named DMC). To support the understanding of the larvicidal action of these formulations, Raman micro-spectroscopy was employed. We also studied the morphological changes in Danio rerio (Zebrafish) gills, a non-target organism, and demonstrate that this is an environmentally friendly approach. Both SC and DMC presented a high photo-larvicidal potential. DMC showed the highest larval mortality, with LC values between 0.01 and 0.02 mg.L. DMC also significantly decreased egg hatchability, reaching a hatching rate of 10 % at 100 mg.L. The analysis of molecular mechanisms via Raman micro-spectroscopy showed that DMC is highly permeable to the peritrophic membrane of the larva, causing irreversible damage to the simple columnar epithelium of the digestive tube. Histological changes found in the D. rerio gills were of minimal or moderate pathological importance, indicating an adaptive trait rather than detrimental characteristics. These findings indicate that curcumin in sugar formulations is highly efficient, especially DMC, proving it to be a promising and safe alternative to control Aedes mosquitoes. Moreover, Raman micro-spectroscopy demonstrated high potential as an analytical technique to understand the mechanism of action of larvicides.
对抗埃及伊蚊媒介仍然是控制许多虫媒病毒传播的关键，如登革热、寨卡病毒和基孔肯雅热。由于对埃及伊蚊有效的控制产品较少，寻找新策略已变得至关重要。强烈鼓励使用具有光动力活性的物质，如姜黄素及其制剂，因为它们具有多靶点作用机制。在本研究中，我们评估了姜黄素在蔗糖（命名为SC）和D-甘露糖醇（命名为DMC）存在下的光杀幼虫和杀卵活性。为了支持对这些制剂杀幼虫作用的理解，采用了拉曼显微光谱法。我们还研究了非靶标生物斑马鱼鳃的形态变化，并证明这是一种环境友好的方法。SC和DMC都具有很高的光杀幼虫潜力。DMC显示出最高的幼虫死亡率，LC值在0.01至0.02mg/L之间。DMC还显著降低了卵的孵化率，在100mg/L时孵化率达到10%。通过拉曼显微光谱法对分子机制的分析表明，DMC对幼虫的围食膜具有高度渗透性，对消化管的单层柱状上皮造成不可逆损伤。在斑马鱼鳃中发现的组织学变化具有最小或中等的病理学重要性，表明这是一种适应性特征而非有害特征。这些发现表明，糖制剂中的姜黄素，尤其是DMC，非常有效，证明它是控制埃及伊蚊的一种有前途且安全的替代方法。此外，拉曼显微光谱法作为一种理解杀幼虫剂作用机制的分析技术显示出很高的潜力。
