Lu Junjia, Liu Jian, Li Huali, Sun Yajiao, Liu Shuwen, Wang Mengyao, Li Yonghe
College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, China.
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China.
Microorganisms. 2025 Mar 21;13(4):709. doi: 10.3390/microorganisms13040709.
has caused significant damage to pine forests, becoming a major biological disaster that hinders the sustainable development of forestry in China. To investigate the efficacy of biological control measures, entomopathogenic fungi were isolated and purified from the larvae of that had succumbed to diseases. The pathogenic capacity of strains was assessed using bioassay methods, and their infection process was observed using scanning electron microscopy. ITS, LSU, and TEF analysis disclosed as the highly virulent strain responsible for the death of . The optimal medium for its mycelial growth and sporulation was found to be PPDA. In addition, the bioassay revealed that the median lethal time (LT) for was 24.34 h and median lethal concentration (LC) was 2.35 × 10 conidia/mL, indicating that possesses potent virulence and demonstrates rapid pathogenicity. Furthermore, scanning electron microscopy demonstrated that initially entered the body of through the spiracle and progressively made its way into the body wall, resulting in the insect's death. The mode of infection for is exceedingly rare. As a consequence, the results of this study can serve as a reference for the management of chewing insects, such as .
已对松林造成了严重破坏，成为阻碍中国林业可持续发展的重大生物灾害。为了研究生物防治措施的效果，从死于疾病的幼虫中分离并纯化了昆虫病原真菌。使用生物测定方法评估菌株的致病能力，并使用扫描电子显微镜观察其感染过程。ITS、LSU和TEF分析表明， 是导致 死亡的高毒力菌株。发现其菌丝生长和产孢的最佳培养基是PPDA。此外，生物测定显示， 的半数致死时间（LT）为24.34小时，半数致死浓度（LC）为2.35×10分生孢子/毫升，表明 具有强大的毒力且致病迅速。此外，扫描电子显微镜显示， 最初通过气门进入 的体内，并逐渐进入体壁，导致昆虫死亡。 的感染方式极为罕见。因此，本研究结果可为诸如 等咀嚼式昆虫的防治提供参考。
