Li Xue-Jiao, Dong Jian-Wei, Cai Le, Mei Rui-Feng, Ding Zhong-Tao
Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China.
Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China.
J Biosci Bioeng. 2017 Nov;124(5):493-497. doi: 10.1016/j.jbiosc.2017.05.012. Epub 2017 Jun 13.
Illigera henryi, an endemic traditional Chinese medicine, contains abundant aporphine alkaloids that possess various bioactivities. In the present study, tubers of I. henryi were fermented by several fungi, and the acetylcholinesterase (AChE) inhibitory activities of non-fermented and fermented I. henryi were measured. The results showed that the fermentation of I. henryi with Clonostachys rogersoniana 828H2 is effective for improving the AChE inhibitory activity. A key biotransformation was found during the C. rogersoniana fermentation for clarifying the improvement of the AChE inhibitory activity of I. henryi: (S)-actinodaphnine (1) was converted to a new 4-hydroxyaporphine alkaloid (4R,6aS)-4-hydroxyactinodaphnine (2) that possessed a stronger AChE inhibitory activity, with an IC value of 17.66±0.06 μM. This paper is the first to report that the pure strain fermentation processing of I. henryi and indicated C. rogersoniana fermentation might be a potential processing method for I. henryi.
中华青牛胆是一种中国特有的传统中药，含有丰富的具有多种生物活性的阿朴啡生物碱。在本研究中，用几种真菌对中华青牛胆的块茎进行发酵，并测定了未发酵和发酵后的中华青牛胆的乙酰胆碱酯酶（AChE）抑制活性。结果表明，用罗氏柱孢828H2对中华青牛胆进行发酵可有效提高其AChE抑制活性。在罗氏柱孢发酵过程中发现了一个关键的生物转化过程，以阐明中华青牛胆AChE抑制活性提高的原因：（S）-莲叶桐碱（1）转化为一种新的4-羟基阿朴啡生物碱（4R,6aS）-4-羟基莲叶桐碱（2），其具有更强的AChE抑制活性，IC值为17.66±0.06 μM。本文首次报道了中华青牛胆的纯菌株发酵工艺，并表明罗氏柱孢发酵可能是中华青牛胆一种潜在的加工方法。
