Tamta Abhishek Kumar, Pandey Renu, Sharma Jiten R, Rai Rajnish, Barman Mritunjoy, M G Deeksha, Mitra Debasis, Mohapatra Pradeep Kumar Das, Sami Rokayya, Al-Mushhin Amina A M, Baakdah Fadi, Mostafa Yasser S, Alrumman Sulaiman A, Helal Mahmoud
Department of Entomology, College of Agriculture, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar 263145, India.
School of Life Sciences, Central University of Gujarat, Gandhinagar 382030, India.
Pathogens. 2022 Nov 30;11(12):1447. doi: 10.3390/pathogens11121447.
Mango hopper ( Lethierry) causes devastations in the early vegetative stage of the mango crop. The classical management of mango hopper is with systemic insecticides but their overuse has caused environmental pollution. Here, we have evaluated the entomopathogenic role of through bioassay and optimized media for its large-scale culturing. The current study reveals the potentiality of as entomopathogenic on . Initially, morphological and molecular characterization was used to validate local isolates' identity as . Further, we have evaluated the entomopathogenic role of through a bioassay, where the highest mean mortality in was observed at a treatment concentration of 3 × 10 conidia/mL, with 96.67% mortality after 168 h of infection. This work also provides insight into the laboratory-based media standardization for , resulting in oatmeal agar media and broth as the most suitable artificial media, and 20 °C temperature for its mass culture. Thus, is a novo-entomopathogenic fungus on and has a high potency to be included in the management of mango hopper pests.
芒果叶蝉（勒蒂埃里）在芒果作物营养生长早期造成严重破坏。芒果叶蝉的传统防治方法是使用内吸性杀虫剂，但过度使用已造成环境污染。在此，我们通过生物测定评估了[某种真菌名称未给出]的昆虫病原作用，并优化了其大规模培养的培养基。当前研究揭示了[该真菌名称]对芒果叶蝉的昆虫病原潜力。最初，通过形态学和分子特征鉴定来验证当地分离株作为[该真菌名称]的身份。此外，我们通过生物测定评估了[该真菌名称]的昆虫病原作用，在处理浓度为3×10分生孢子/毫升时，观察到最高平均死亡率，感染168小时后死亡率为96.67%。这项工作还为基于实验室的[该真菌名称]培养基标准化提供了见解，结果表明燕麦片琼脂培养基和肉汤是最适合的人工培养基，大规模培养的温度为20℃。因此，[该真菌名称]是一种对芒果叶蝉新的昆虫病原真菌，具有很高的潜力可用于芒果叶蝉害虫的防治。
