Institute for Water and Wastewater Technology, Durban University of Technology, PO Box 1334, Durban 4000, South Africa.
Institute for Water and Wastewater Technology, Durban University of Technology, PO Box 1334, Durban 4000, South Africa.
Bioresour Technol. 2014 Sep;168:127-35. doi: 10.1016/j.biortech.2014.03.064. Epub 2014 Mar 27.
The potential of nitrogen sources supplementing domestic wastewater for the cultivation of microalgae was assessed. Urea, potassium nitrate, sodium nitrate and ammonium nitrate were evaluated for their effect on cultivation and lipid production of Chlorella sorokiniana. Urea showed the highest biomass yield of 0.220 g L(-1) and was selected for further experimentation. Urea concentrations (0-10 g L(-1)) were assessed for their effect on growth and microalgal physiology using pulse amplitude modulated fluorometry. A concentration of 1.5 g L(-1) urea produced 0.218 g L(-1) biomass and 61.52% lipid by relative fluorescence. Physiological stress was evident by the decrease in relative Electron Transport Rate from 10.45 to 6.77 and quantum efficiency of photosystem II charge separation from 0.665 to 0.131. Gas chromatography analysis revealed that C16:0, C18:0, C18:1, C18:2 and C18:3 were the major fatty acids produced by C. sorokiniana. Urea proved to be an effective nitrogen supplement for cultivation of C. sorokiniana in wastewater.
评估了补充生活污水中氮源用于培养微藻的潜力。评估了尿素、硝酸钾、硝酸钠和硝酸铵对小球藻培养和产脂的影响。尿素表现出最高的生物量产量为 0.220 g/L，并被选为进一步实验的选择。使用脉冲幅度调制荧光法评估了 0-10 g/L 的尿素浓度对生长和微藻生理的影响。1.5 g/L 尿素产生 0.218 g/L 生物质和 61.52%的相对荧光脂质。相对电子传递率从 10.45 下降到 6.77，光系统 II 电荷分离的量子效率从 0.665 下降到 0.131，表明存在生理应激。气相色谱分析表明，C16:0、C18:0、C18:1、C18:2 和 C18:3 是小球藻产生的主要脂肪酸。尿素被证明是在废水中培养小球藻的有效氮补充物。
