Cirio Silvia, Salerno Claudia, Guglielmetti Simone Domenico, Mezzasalma Valerio, Sarrica Andrea, Kirika Natalja, Campus Guglielmo, Cagetti Maria Grazia
Department of Biomedical, Surgical and Dental Sciences, University of Milan, Via Beldiletto 1, 20142 Milan, Italy.
Department of Restorative, Preventive and Pediatric Dentistry, University of Bern, Freiburgstrasse 7, 3012 Bern, Switzerland.
Microorganisms. 2025 Mar 24;13(4):721. doi: 10.3390/microorganisms13040721.
Probiotics are increasingly used to promote oral health, with demonstrating proven effectiveness. Additionally, shows promising potential in this field. Chewing gum has recently been proposed as an innovative delivery method for probiotics. This study aimed to evaluate the kinetics in saliva of and in microencapsulated and non-microencapsulated forms (LGG) following their administration via sugar-free chewing gums. A randomized cross-over trial was conducted involving 10 volunteers. Participants chewed gums containing one of the probiotic strains for 10 min. Saliva samples were collected at baseline (T) and six subsequent time points over 2 h (T-T). Colony-forming units (CFUs) were identified and quantified. The Tukey's range test was applied to make pairwise comparisons between different probiotics at every time point, between different time points of the same probiotic, and between the area under the curve describing the kinetics of different probiotics in saliva. At T, all probiotics exhibited peak counts, followed by a gradual decline until T. SNZ1969 achieved the highest counts at T, T, and T (mean log CFU/mL: 6.1 ± 0.5; 5.8 ± 0.5; 5.6 ± 0.5, respectively), while the non-microencapsulated form of LGG peaked at T, T, and T (mean log CFU/mL: 4.0 ± 0.7; 3.8 ± 0.9; 3.3 ± 1.3, respectively). The participants reported no adverse effects. Probiotics were detectable in saliva up to 2 h post-administration via chewing gum, indicating its suitability as a delivery vehicle. However, significant variability was observed among participants.
益生菌越来越多地被用于促进口腔健康，且已证明具有有效性。此外，[此处原文缺失具体内容]在该领域显示出有前景的潜力。口香糖最近被提议作为益生菌的一种创新递送方式。本研究旨在评估通过无糖口香糖给药后，微胶囊化和非微胶囊化形式的[此处原文缺失具体内容]和鼠李糖乳杆菌（LGG）在唾液中的动力学。进行了一项随机交叉试验，涉及10名志愿者。参与者咀嚼含有其中一种益生菌菌株的口香糖10分钟。在基线（T0）和随后2小时内的六个时间点（T1 - T6）收集唾液样本。鉴定并定量菌落形成单位（CFU）。应用Tukey范围检验在每个时间点对不同益生菌之间、同一益生菌的不同时间点之间以及描述唾液中不同益生菌动力学的曲线下面积之间进行两两比较。在T0时，所有益生菌均呈现计数峰值，随后逐渐下降直至T6。SNZ1969在T1、T3和T5时达到最高计数（平均log CFU/mL分别为：6.1±0.5；5.8±0.5；5.6±0.5），而未微胶囊化形式的LGG在T1、T3和T5时达到峰值（平均log CFU/mL分别为：4.0±0.7；3.8±0.9；3.3±1.3）。参与者报告无不良反应。通过口香糖给药后2小时内唾液中均可检测到益生菌，表明其适合作为递送载体。然而，参与者之间观察到显著的变异性。
