Ma Wenyao, Han Zhe, Liu Xinlei, Cui Weipeng, Zhen Dongyu, Zhou Xiaolu, Song Yuan, Jiang Shuaiming
Key Laboratory of Food Nutrition and Functional Food of Hainan Province, School of Food Science and Engineering, Hainan University, Haikou 570228, China.
Microorganisms. 2025 Mar 25;13(4):731. doi: 10.3390/microorganisms13040731.
The intestinal tract extends several times the length of bodies, with varying environmental conditions across different segments (small intestinal and large intestinal), thereby harboring distinct gut microbiota. Most studies focused on the quantitative responses of gut microbiota upon probiotics entering the gut, without an in-depth analysis of how the genetic change in local gut microbiota. Therefore, in this experiment, C57BL/6J male mice were once administered HNU082 (Lp082). Then, the mice were euthanized on the 1st, 3rd, and 7th days after gavage, and the contents of the small and large intestines of the mice were scraped for metagenomic analysis. Based on the characterization of large intestine and small intestine bacteria, changes in the diversity and abundance of single-nucleotide variants (SNVs) of microbiota were analyzed. There were observable distinct responses at the genetic level. A significant number of SNVs were identified in in the large intestine. These SNVs may impact the utilization of carbohydrates in . Ingested probiotics traversed the entire gut and interacted with the indigenous microbiota, driving the evolution of the indigenous gut microbiota in the different intestinal segments, thereby influencing microbial growth and metabolism. This study investigates the role of probiotics in the evolution of gut microbiota. It offers new probiotic insights and a basis for targeted interventions.
肠道的长度是身体长度的数倍，不同节段（小肠和大肠）的环境条件各异，因而拥有不同的肠道微生物群。大多数研究聚焦于益生菌进入肠道后肠道微生物群的定量反应，而未深入分析局部肠道微生物群的基因变化情况。因此，在本实验中，对C57BL/6J雄性小鼠一次性给予HNU082（Lp082）。然后，在灌胃后的第1天、第3天和第7天对小鼠实施安乐死，并刮取小鼠小肠和大肠内容物进行宏基因组分析。基于大肠和小肠细菌的特征，分析了微生物群单核苷酸变异（SNV）的多样性和丰度变化。在基因水平上观察到了明显不同的反应。在大肠中鉴定出了大量的SNV。这些SNV可能会影响[此处原文缺失相关内容]中碳水化合物的利用。摄入的益生菌穿过整个肠道并与本土微生物群相互作用，推动不同肠道节段中本土肠道微生物群的进化，从而影响微生物的生长和代谢。本研究探讨了益生菌在肠道微生物群进化中的作用。它为靶向干预提供了新的益生菌见解和依据。
