Gouw Boyd H T, Oliveira Flavia C M, Kooistra Hans S, Spee Bart, van Uden Lisa, Penning Louis C
Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, The Netherlands.
Health and Animal Production in Amazônia Program, Universidade Federal Rural da Amazônia, Belém 66077-830, Brazil.
Vet Sci. 2025 Apr 13;12(4):362. doi: 10.3390/vetsci12040362.
Diabetes mellitus (DM) is a common potentially life-threatening endocrine disorder in pets and humans. Since only symptomatic treatment is available, a more sustainable treatment is urgently needed.
The aim of this study is to establish functional differentiated canine pancreatic β-cells that release insulin upon glucose stimulus.
Pancreatic tissue was obtained from surplus material of healthy dogs (n = 4), euthanized for non-pancreatic related research. Ductal cells were isolated and expanded in dog pancreas expansion media (dpEM) and differentiated and maturated in five sequentially added pancreas differentiation media (PDMs). Gene expression was analyzed by reversed transcriptase qPCR (RT-qPCR), and insulin release was analyzed with a canine-specific ELISA.
Canine pancreatic ductal cells ( and expression) were differentiated into β-cells expressing key β-cell-related genes: , and low levels of . Neither (α-cells) nor and were expressed, and was expressed at low levels. The differentiated cells released insulin upon glucose stimulation.
The step-by-step differentiation protocol, mimicking pancreatic organogenesis, resulted in β-cells secreting insulin levels suitable for β-cell disease modelling. It remains to be seen if stem cells from diseased animals behave similarly.
糖尿病（DM）是宠物和人类中一种常见的、可能危及生命的内分泌疾病。由于目前仅有对症治疗方法，因此迫切需要更具可持续性的治疗手段。
本研究旨在建立在葡萄糖刺激下能释放胰岛素的功能性分化犬胰腺β细胞。
胰腺组织取自健康犬（n = 4）的剩余材料，这些犬因非胰腺相关研究而实施安乐死。分离导管细胞并在犬胰腺扩增培养基（dpEM）中进行扩增，然后在依次添加的五种胰腺分化培养基（PDM）中进行分化和成熟培养。通过逆转录定量聚合酶链反应（RT-qPCR）分析基因表达，并使用犬特异性酶联免疫吸附测定（ELISA）分析胰岛素释放情况。
犬胰腺导管细胞（以及 表达）分化为表达关键β细胞相关基因的β细胞： 、 和低水平的 。既未检测到 （α细胞）表达，也未检测到 和 表达，且 表达水平较低。分化后的细胞在葡萄糖刺激下释放胰岛素。
模仿胰腺器官发生的逐步分化方案产生了分泌胰岛素水平适合用于β细胞疾病建模的β细胞。患病动物的干细胞是否表现出类似行为还有待观察。
