Ali Sawsan A, Al-Mousawi Zainab A H, Al-Rikaby Ahlam A, Farouk Sameh Mohamed, Elnesr Shaaban S
Department of Anatomy and Histology, College of Veterinary Medicine, University of Basrah, Basrah 61004, Iraq.
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Basrah, Basrah 61004, Iraq.
Vet Sci. 2025 Apr 15;12(4):371. doi: 10.3390/vetsci12040371.
This study aimed to investigate the effect of extract on testicular dysfunction in rats and explain the involvement of the androgen receptor signaling pathway as a biomarker in maintaining fertility during sodium fluoride (NaF) treatment. Thirty-two male rats were divided equally into four groups and received treatment for 60 days. The control group (I) received normal saline; group II received Artemisia extract at 100 mg/kg b.w.; group III received NaF at 12 mg/kg b.w. orally; and group IV received NaF (12 mg/kg b.w.) and Artemisia extract (100 mg/kg b.w.). The testis weights and the lipid peroxidation, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone levels were estimated. The genital organs were prepared and immunoreacted with a receptor. Histomorphometric analyses were performed to obtain the diameter of the seminiferous tubules and the height of the germinal epithelia in the testes. The results showed that exposure to NaF caused a significant increase in testis weight and malondialdehyde (MDA) and a decrease in serum LH, FSH, and testosterone concentrations compared to the control group, while extract administration induced an increase in the levels of these hormones in group IV. Testicular histological and histomorphometric changes were observed in group III: degenerative seminiferous tubules with vascular congestion, disorganization of the germinal layer, and decreased seminiferous tubule diameter and germinal epithelium height. The expression of androgen receptors in the testes of the NaF-treated rats was significantly reduced. In contrast, these testicular histological changes were ameliorated in rats treated with the extract. The results allow us to conclude that the administration of confers positive effects on male reproductive function by inhibiting fluoride, maybe via ameliorative testicular function.
本研究旨在探讨[提取物名称]提取物对大鼠睾丸功能障碍的影响，并解释雄激素受体信号通路作为生物标志物在氟化钠（NaF）治疗期间维持生育能力中的作用。将32只雄性大鼠平均分为四组，进行60天的治疗。对照组（I）给予生理盐水；第二组给予100mg/kg体重的青蒿提取物；第三组口服12mg/kg体重的NaF；第四组给予NaF（12mg/kg体重）和青蒿提取物（100mg/kg体重）。测定睾丸重量、脂质过氧化、黄体生成素（LH）、卵泡刺激素（FSH）和睾酮水平。制备生殖器官并与受体进行免疫反应。进行组织形态计量学分析以获得睾丸中曲细精管的直径和生精上皮的高度。结果表明，与对照组相比，暴露于NaF导致睾丸重量和丙二醛（MDA）显著增加，血清LH、FSH和睾酮浓度降低，而给予提取物使第四组这些激素水平升高。在第三组中观察到睾丸组织学和组织形态计量学变化：曲细精管退行性变伴血管充血、生发层紊乱、曲细精管直径和生精上皮高度降低。NaF处理大鼠睾丸中雄激素受体的表达显著降低。相反，用提取物处理的大鼠这些睾丸组织学变化得到改善。结果使我们得出结论，[提取物名称]提取物的给药可能通过改善睾丸功能抑制氟，从而对雄性生殖功能产生积极影响。
