Chen Wei, Hu Yanqing, Qin Yan, Li Yuying, Zhang Xinyu, Huang Haixin, Liu Mengjia, Zheng Yuping, Lu Xuelian, Wang Qiaoqiong, Yang Jianuo, Kang Liting, Xie Lulu, Zhao Baopeng, Lan Tian, Sun Wenchao
Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou 325035, China.
Viruses. 2025 Apr 8;17(4):543. doi: 10.3390/v17040543.
Porcine parvovirus 8 (PPV8), the most recently discovered PPV genotype, was first reported in pigs in Guangdong, China, in 2021. In this study, we assessed 69 lung tissue samples collected from animals with high fever or respiratory syndrome on pig farms in Guangxi in 2018. Five nearly full-length genome sequences were characterized and analyzed. The percentage of PPV8-positive samples was 13.04% (9/69), and five complete PPV8 sequences were obtained, which were designated PPV8-A, PPV-B, PPV8-G, PPV8-H, and PPV8-I. The genomic sequence homology among the five PPV8 strains and other PPVs was 25.91-98.84%, with the closest genetic relationship to PPV8-GDJM2021 (98.84%), followed by PPV1 (44.64%). For the NS1 protein, phylogenetic analysis revealed that the identified PPV8-I was closely related to PPV8-GDJM2021 and that PPV8-A was closely related to PPV8-H, whereas PPV8-B and PPV8-G were more distantly related to the other PPV8 strains. For the VP1 protein, phylogenetic analysis revealed a close correlation between PPV8-H and PPV8-GMJM2021, whereas PPV8-A, PPV8-B, PPV8-G, and PPV8-I were more distantly related. In conclusion, five nearly full-length sequences were amplified, and the molecular characteristics of PPV8 were analyzed. These findings improve our understanding of the PPV8 genome.
猪细小病毒8型（PPV8）是最近发现的PPV基因型，于2021年首次在中国广东的猪中报道。在本研究中，我们评估了2018年从广西猪场高热或呼吸道综合征动物采集的69份肺组织样本。对5个近乎全长的基因组序列进行了特征分析。PPV8阳性样本的百分比为13.04%（9/69），获得了5个完整的PPV8序列，分别命名为PPV8-A、PPV-B、PPV8-G、PPV8-H和PPV8-I。这5个PPV8毒株与其他PPV的基因组序列同源性为25.91%-98.84%，与PPV8-GDJM2021的遗传关系最密切（98.84%），其次是PPV1（44.64%）。对于NS1蛋白，系统发育分析表明，鉴定出的PPV8-I与PPV8-GDJM2021密切相关，PPV8-A与PPV8-H密切相关，而PPV8-B和PPV8-G与其他PPV8毒株的关系较远。对于VP1蛋白，系统发育分析表明PPV8-H与PPV8-GMJM2021密切相关，而PPV8-A、PPV8-B、PPV8-G和PPV8-I的关系较远。总之，扩增了5个近乎全长的序列，并分析了PPV8的分子特征。这些发现增进了我们对PPV8基因组的了解。
