Department of Animal Biotechnology, Faculty of Basic Sciences, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600 007, India.
Sree Sastha Institute of Engineering and Technology, Chennai, India.
Trop Anim Health Prod. 2022 Jun 2;54(3):195. doi: 10.1007/s11250-022-03194-8.
This study used 56 aborted and stillborn fetuses from organized swine farms in Tamil Nadu and Kerala, southern states of India. All samples were screened by using a PCR assay that targets the NS1 gene for PPV. Furthermore, the PCR positive samples were subjected to amplification of the VP2 gene of PPV1 with designed primers and sequenced for further study. The PCR screening of 56 samples found that 14.3% (n = 8) were positive for PPV genome. According to VP2 gene-based PCR for PPV1, 897 bp specific amplicons were detected in all eight of the samples. Two of the eight positive samples (L17 and T5) were sequenced and annotated randomly. The BLAST analysis of contig sequence INDTNCHN-T5 revealed 100% sequence homology with Chinese PPV1genome, whereas sequence from INDTNCHN-L17 revealed 99.43% sequence homology with Spain, Chinese, and German. PPV1 sequences and both the sequences INDTNCHN-T5 and INDTNCHN-L17 were submitted to the GenBank under the accession numbers MW822566 and MW822567 respectively. A phylogenetic analysis of the sequences in this study revealed specific grouping along with PPV1 strains in cluster E. Amino acid analysis of both isolated sequences in addition to the reference sequence from PPV1 showed variations in position 215 (I to T) in both the isolates, variation at position 228 (Q to E) in T5 isolate and variations at position 59 (L to M) and 314 (K to E) in L17 isolate. This study represents the first report of PPV1 cluster E in Tamil Nadu, southern India.
本研究使用了来自印度南部泰米尔纳德邦和喀拉拉邦的组织化养猪场的 56 例流产和死胎胎儿。所有样本均通过针对 PPV 的 NS1 基因的 PCR 检测进行了筛查。此外，对 PCR 阳性样本进行了设计引物的 PPV1 VP2 基因扩增，并对进一步研究进行了测序。对 56 个样本的 PCR 筛查发现，14.3%（n=8）的样本 PPV 基因组呈阳性。根据针对 PPV1 的 VP2 基因的 PCR，在所有 8 个样本中均检测到 897bp 的特异性扩增子。在 8 个阳性样本中，有 2 个（L17 和 T5）进行了测序并随机注释。INDTNCHN-T5 的拼接序列的 BLAST 分析显示，与中国 PPV1 基因组的序列同源性为 100%，而 INDTNCHN-L17 的序列与西班牙、中国和德国的序列同源性为 99.43%。PPV1 序列以及 INDTNCHN-T5 和 INDTNCHN-L17 的序列均已提交给 GenBank，登录号分别为 MW822566 和 MW822567。对本研究中序列的系统发育分析表明，它们与聚类 E 中的 PPV1 株特异性聚集。对两个分离株的氨基酸分析以及来自 PPV1 的参考序列显示，两个分离株在位置 215（I 到 T）都有变异，T5 分离株在位置 228（Q 到 E）有变异，L17 分离株在位置 59（L 到 M）和 314（K 到 E）有变异。本研究首次报道了印度南部泰米尔纳德邦的 PPV1 聚类 E。
