Enhanced Compressive Strength of PVA/SA Composite Hydrogel by Highly Dispersed Hydroxyapatite Nanofibers.

Rapid functional soft tissue restoration has shown considerable promise as a framework for stability and coordination in the human body. Inspired by the anisotropic arrangement of structures with soft and hard phases in biological tissues, such as tendon, cartilage, and ligament, many methods have been used to fabricate composite hydrogels with appropriate mechanical properties. The development of a high-strength hydrogel with strong bioactivity remains a key barrier to replace soft tissues with comparable synthetic structures. In this study, a highly dispersed hydroxyapatite nanofiber (HANF) reinforced polyvinyl alcohol-sodium alginate (PVA/SA) composite hydrogel is prepared for soft tissue replacement. The effect of the addition of HANF on the microstructure and properties of composite hydrogel is also investigated. The results show that the PVA/SA hydrogel, after the incorporation of HANF, combines well with the PVA/SA hydrogel (HANF@PVA/SA). SEM morphologies show that dispersed HANF can enter the holes of the three-dimensional structure of the composite hydrogel. Additionally, the addition of HANF can enhance the compressive strength of the PVA/SA composite hydrogel from 4.66 MPa to 7.72 MPa. At the same time, the HANF@PVA/SA hydrogel maintains the same excellent hydrophilicity as the original PVA/SA hydrogel. Finally, cytotoxicity and live/dead cell staining tests also confirmed its excellent biocompatibility, demonstrating its tremendous potential for use in soft tissue repair.