Pachas W N, Schor M, Aulakh G S
Diagn Microbiol Infect Dis. 1985 Jul;3(4):295-309. doi: 10.1016/0732-8893(85)90004-5.
L-forms from a Corynebacterium were induced in hyperosmolar media and gradually adapted to normal osmolarity over a period of two years. During this adaptation several morphologic L-form variants derived from the L-form cultures and were serologically identified as Acholeplasma laidlawii A. The possibility that the bacterial and L-form cultures were contaminated with acholeplasmas was carefully investigated; this was determined not to be the case. Membrane protein gel electrophoresis patterns of these L-form variants were identical to the ATCC A. laidlawii strain PG-8. Acholeplasma phage MVL-1 displayed no affinity for these L-form variants. Phage MVL-2 showed low affinity, but after virus enhancement in the specific host, high plaquing efficiency ensued. DNA hybridization experiments showed a high level of nucleotide sequence homology (greater than 90%) between the L-form-derived variants and PG-8. The homology between the diphtheroid L-forms and the PG-8 strain was 16.4% with te50 values of 86%; this indicates strong base pairing homology. These findings suggest that the L-form variants are acholeplasmas and that they are biologically and genetically related to the Corynebacterium L-forms.
在高渗培养基中诱导出棒状杆菌的L型，并在两年时间内逐渐适应正常渗透压。在这一适应过程中，从L型培养物中衍生出几种形态学上的L型变体，并通过血清学鉴定为莱氏无胆甾原体A。仔细研究了细菌和L型培养物被无胆甾原体污染的可能性；确定情况并非如此。这些L型变体的膜蛋白凝胶电泳图谱与美国典型培养物保藏中心（ATCC）的莱氏无胆甾原体PG - 8菌株相同。无胆甾原体噬菌体MVL - 1对这些L型变体没有亲和力。噬菌体MVL - 2显示出低亲和力，但在特定宿主中病毒增强后，出现了高噬斑形成效率。DNA杂交实验表明，L型衍生变体与PG - 8之间存在高水平的核苷酸序列同源性（大于90%）。类白喉L型与PG - 8菌株之间的同源性为16.4%，te50值为86%；这表明存在强碱基配对同源性。这些发现表明，L型变体是无胆甾原体，并且它们在生物学和遗传学上与棒状杆菌L型相关。
