Hood R L, Beitz D C, Johnson D C
Comp Biochem Physiol B. 1985;81(3):667-70. doi: 10.1016/0305-0491(85)90383-9.
To study the pathway of lactate utilization as a carbon source for fatty acid synthesis, the effect of (-)-hydroxycitrate, agaric acid, sodium oxamate, 2-n-butyl malonate and alpha-cyano-4-hydroxycinnamate on the rate of in vitro conversion of lactate, acetate and glucose to fatty acids was measured in bovine and rat adipose tissues. Sodium oxamate and hydroxycitrate caused less fatty acid to be synthesized from lactate in bovine adipose tissue. Hydroxycitrate depressed fatty acid synthesis from glucose in rat adipose tissue. alpha-Cyano-4-hydroxycinnamate was an effective inhibitor of lipogenesis from all substrates and may act as a specific inhibitor in adipose tissue. Although the inhibitors were absorbed poorly into adipocytes, the results indicate that conversion of lactate to fatty acids probably occurs by way of the citrate cleavage pathway.
为研究乳酸作为脂肪酸合成碳源的利用途径，测定了（-）-羟基柠檬酸、木耳酸、草酸钠、2-正丁基丙二酸和α-氰基-4-羟基肉桂酸对牛和大鼠脂肪组织中乳酸、乙酸和葡萄糖体外转化为脂肪酸速率的影响。草酸钠和羟基柠檬酸使牛脂肪组织中由乳酸合成的脂肪酸减少。羟基柠檬酸抑制大鼠脂肪组织中由葡萄糖合成脂肪酸。α-氰基-4-羟基肉桂酸是所有底物脂肪生成的有效抑制剂，可能在脂肪组织中作为特异性抑制剂起作用。尽管抑制剂进入脂肪细胞的吸收率很低，但结果表明乳酸转化为脂肪酸可能通过柠檬酸裂解途径发生。
