Zhang Jing, Shao Wenna, Xu Yongxin, Tian Fa'an, Chen Jinchao, Wang Dongzhi, Lin Xuelei, He Chongsheng, Yang Xiaofei, Staiger Dorothee, Ding Yiliang, Yu Xiang, Xiao Jun
Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
Nat Commun. 2025 Apr 26;16(1):3947. doi: 10.1038/s41467-025-59329-6.
Abscisic acid (ABA) is a crucial phytohormone involved in plant growth and stress responses. While the transcriptional regulation triggered by ABA is well-documented, its effects on translational regulation have been less studied. Through Ribo-seq and RNA-seq analyses, we find that ABA treatment not only influences gene expression at the mRNA level but also significantly impacts mRNA translation efficiency (TE) in Arabidopsis thaliana. ABA inhibits global mRNA translation via its core signaling pathway, which includes ABA receptors, protein phosphatase 2Cs (PP2Cs), and SNF1-related protein kinase 2 s (SnRK2s). Upon ABA treatment, Glycine-rich RNA-binding proteins 7 and 8 (GRP7&8) protein levels decrease due to both reduced mRNA level and decreased TE, which diminishes their association with polysomes and leads to a global decline in mRNA TE. The absence of GRP7&8 results in a global impairment of ABA-regulated translational changes, linking ABA signaling to GRP7-dependent modulation of mRNA translation. The regulation of GRP7 on TE relies significantly on its direct binding to target mRNAs. Moreover, mRNA translation efficiency under drought stress is partially dependent on the ABA-GRP7&8 pathways. Collectively, our study reveals GRP7's role downstream of SnRK2s in mediating translation regulation in ABA signaling, offering a model for ABA-triggered multi-route regulation of environmental adaptation.
脱落酸（ABA）是一种参与植物生长和应激反应的关键植物激素。虽然由ABA触发的转录调控已有充分记录，但其对翻译调控的影响研究较少。通过核糖体测序（Ribo-seq）和RNA测序（RNA-seq）分析，我们发现ABA处理不仅影响拟南芥中mRNA水平的基因表达，还显著影响mRNA翻译效率（TE）。ABA通过其核心信号通路抑制整体mRNA翻译，该通路包括ABA受体、蛋白磷酸酶2C（PP2C）和SNF1相关蛋白激酶2（SnRK2）。ABA处理后，富含甘氨酸的RNA结合蛋白7和8（GRP7&8）的蛋白水平由于mRNA水平降低和TE降低而下降，这减少了它们与多核糖体的结合，并导致mRNA TE整体下降。GRP7&8的缺失导致ABA调节的翻译变化整体受损，将ABA信号传导与mRNA翻译的GRP7依赖性调节联系起来。GRP7对TE的调节在很大程度上依赖于其与靶mRNA的直接结合。此外，干旱胁迫下的mRNA翻译效率部分依赖于ABA-GRP7&8通路。总体而言，我们的研究揭示了GRP7在SnRK2下游在介导ABA信号传导中的翻译调控中的作用，为ABA触发的环境适应多途径调节提供了一个模型。
