Zhang Jing, Xu Yongxin, Xiao Jun
Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Sci Data. 2024 Dec 28;11(1):1447. doi: 10.1038/s41597-024-04324-7.
Abscisic acid (ABA) is a crucial phytohormone that regulates plant growth and stress responses. While substantial knowledge exists about transcriptional regulation, the molecular mechanisms underlying ABA-triggered translational regulation remain unclear. Recent advances in deep sequencing of ribosome footprints (Ribo-seq) enable the mapping and quantification of mRNA translation efficiency. Additionally, RNA-binding proteins (RBPs) play essential roles in translational regulation by interacting with target RNA molecules, making the identification of binding sites via UV crosslinking and immunoprecipitation (CLIP) critical for understanding RBP function. Glycine-rich RNA-binding proteins (GRPs), a prominent class of RBPs in plants, are responsive to ABA. In this study, RNA-seq and Ribo-seq analyses were conducted on 3-day-old Col-0 and grp7grp8 seedlings of Arabidopsis thaliana, treated with either ABA or mock solutions. These analyses facilitated deep sequencing of total mRNA and mRNA fragments protected by translating ribosomes. Additionally, CLIP-seq analysis of pGRP7::GRP7-GFP grp7-1 identified RNA bound by GRP7. This multi-omics dataset allows for a comprehensive investigation of the plant's response to ABA from various perspectives, providing a significant resource for studying ABA-regulated mRNA translation efficiency.
脱落酸（ABA）是一种关键的植物激素，可调节植物生长和应激反应。虽然关于转录调控已有大量知识，但ABA触发的翻译调控的分子机制仍不清楚。核糖体足迹深度测序（Ribo-seq）的最新进展使得能够对mRNA翻译效率进行定位和定量。此外，RNA结合蛋白（RBP）通过与靶RNA分子相互作用在翻译调控中发挥重要作用，因此通过紫外线交联和免疫沉淀（CLIP）鉴定结合位点对于理解RBP功能至关重要。富含甘氨酸的RNA结合蛋白（GRP）是植物中一类重要的RBP，对ABA有反应。在本研究中，对用ABA或模拟溶液处理的3日龄拟南芥Col-0和grp7grp8幼苗进行了RNA测序和Ribo-seq分析。这些分析有助于对总mRNA和由正在翻译的核糖体保护的mRNA片段进行深度测序。此外，对pGRP7::GRP7-GFP grp7-1进行的CLIP-seq分析鉴定了GRP7结合的RNA。这个多组学数据集允许从多个角度全面研究植物对ABA的反应，为研究ABA调节的mRNA翻译效率提供了重要资源。
