Kocemba Marta, Waller Edward, Waker Anthony J
Ontario Tech University, Oshawa, Canada.
Sci Rep. 2025 Apr 26;15(1):14672. doi: 10.1038/s41598-025-99230-2.
This study followed changes in the optical quality of teleost eye-lenses following exposure to non-ionizing UVB radiation and was carried out as an adjunct to a wider ongoing study on the effects of ionizing radiation on vertebrate eye-lenses. The lenses of rainbow trout (Oncorhynchus mykiss) were excised and cultured in vitro, then exposed to 0.2 J cm of UVB radiation. The optical quality of the lenses was measured daily until the end-point of full surface clouding was observed. Optical quality assessment was accomplished through laser focal analysis, which is a method of measuring changes in lens optical function while the lens is still transparent. Lens optical quality is quantified by measuring focal lengths and focal length variability in each lens. Our study found a significant difference in lens quality in irradiated lenses compared to control lenses 11 days after irradiation. Irradiated lenses also showed a reduction in optical quality on the last day of transparency before lens damage progressed to full surface clouding. Irradiated lenses also maintained transparency in culture for longer than control lenses. Therefore, we have shown that UVB decreases the optical quality of vertebrate eye-lenses and potentially induces a stress response in the maintenance of lens transparency.
本研究追踪了硬骨鱼眼晶状体在暴露于非电离紫外线B辐射后的光学质量变化，作为正在进行的关于电离辐射对脊椎动物眼晶状体影响的更广泛研究的辅助部分。虹鳟（Oncorhynchus mykiss）的晶状体被切除并在体外培养，然后暴露于0.2 J/cm²的紫外线B辐射。每天测量晶状体的光学质量，直到观察到完全表面浑浊的终点。光学质量评估通过激光聚焦分析完成，这是一种在晶状体仍透明时测量晶状体光学功能变化的方法。通过测量每个晶状体的焦距和焦距变异性来量化晶状体光学质量。我们的研究发现，照射后11天，与对照晶状体相比，受照射晶状体的质量存在显著差异。在晶状体损伤发展为完全表面浑浊之前的透明度最后一天，受照射晶状体的光学质量也有所下降。受照射晶状体在培养中保持透明的时间也比对照晶状体长。因此，我们表明紫外线B会降低脊椎动物眼晶状体的光学质量，并可能在维持晶状体透明度方面引发应激反应。
