印度东北部锡金邦5岁以下儿童轮状病毒肠胃炎的流行病学与基因分型
Epidemiology and Genotyping of Rotavirus Gastroenteritis in Children <5 Years in Sikkim, North East India.
作者信息
Gurung Shrijana, Chatterjee Ananya, Yonzan Ruth, Majumdar Agniva, Chhophel Tashi Pegey, Tewari Ekta, Sharma Dhruva Kumar, Banu Hasina, Sarkar Mamta Chawla, Dutta Shanta
机构信息
Department of Virology, Sir Thutob Namgyal Memorial Hospital, Gangtok, Sikkim, India.
Regional Viral Research and Diagnostic Laboratory, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India.
出版信息
J Glob Infect Dis. 2025 Feb 24;17(1):24-28. doi: 10.4103/jgid.jgid_91_24. eCollection 2025 Jan-Mar.
INTRODUCTION
Rotavirus is a frequent cause of gastroenteritis in young children. It is seasonal in many countries but occurs year-round in India.Since the launch of the Rotavirus Vaccine (RVV) morbidity and mortality in children have greatly decreased. This study was carried out prior to the inclusion of RVV in the state vaccination schedule in 2019. The objective of the study was to estimate the burden of Rota Virus Gastroeneteritis in kids under 5 years of age, attending a government tertiary care hospital in the state and to identify the common circulating genotypes of Rotavirus.
METHODS
Stool samples from children with more than three episodes of loose stool that were negative for bacterial enteric pathogens were tested by Enzyme Linked Immunosorbent Assay (ELISA) for Rotavirus antigen. Positive stool samples were transported to the Regional Viral Research and Diagnostic Laboratory at National Institute for Cholera and Enteric Diseases for genotyping.
RESULTS
200 stool samples were screened and 40 samples (20%) were positive for Rotavirus antigen by ELISA. G3P [8] - 33% (9/27), followed by G1P[8] -15%(4/27) and G2P4 - 11%( 3/27) were the most common genotypes.
CONCLUSION
Rotavirus is a significant cause of gastroenteritis in children under five years of age in the Indian state of Sikkim.
引言
轮状病毒是幼儿肠胃炎的常见病因。在许多国家，其发病具有季节性，但在印度全年都有发生。自轮状病毒疫苗（RVV）推出以来，儿童的发病率和死亡率大幅下降。本研究在2019年该州将RVV纳入国家疫苗接种计划之前进行。该研究的目的是估计该州一家政府三级护理医院中5岁以下儿童的轮状病毒肠胃炎负担，并确定轮状病毒的常见流行基因型。
方法
对粪便中细菌肠道病原体检测呈阴性且有三次以上腹泻发作的儿童粪便样本进行酶联免疫吸附测定（ELISA），检测轮状病毒抗原。阳性粪便样本被送往国家霍乱和肠道疾病研究所的区域病毒研究和诊断实验室进行基因分型。
结果
共筛查了200份粪便样本，其中40份（20%）通过ELISA检测轮状病毒抗原呈阳性。最常见的基因型是G3P[8] - 33%（9/27），其次是G1P[8] - 15%（4/27）和G2P4 - 11%（3/27）。
结论
在印度锡金邦，轮状病毒是5岁以下儿童肠胃炎的重要病因。
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