Department of Pediatric Medicine, SMS Medical College, Jaipur, Rajasthan, India.
Department of Pathology, SMS Medical College, Jaipur, Rajasthan, India.
Indian J Pediatr. 2021 Mar;88(Suppl 1):90-96. doi: 10.1007/s12098-020-03569-5. Epub 2020 Nov 28.
To estimate the proportion of rotavirus diarrhea among hospitalized children aged under-five years, to determine the circulating rotavirus genotypes and to know impact rotavirus vaccine on prevalence and severity of rotavirus diarrhea.
This study was a hospital based cross-sectional observational study conducted over a period of 29 mo (September 2017 through January 2020). Stool samples were collected from children who fall within the age range of 0-59 mo with acute diarrhea attending emergency or needing admission. Stool samples were tested for rotavirus by the enzyme linked immune-sorbent assay (ELISA) and genotyped using published methods.
Out of 1480 samples, 360 (24.32%) cases were positive for rotavirus by ELISA, majority of them were male (62.97%). Maximum rotavirus positivity was found in the age group of <11 mo (55.27%). Statistically significance difference was seen in episodes of diarrhea and experience of vomiting in rotavirus diarrhea cases. Highest prevalence has been seen during winter season. The most prevalent G and P type combinations were G3P [8] strains [122 (34.08%)], G2P [4] [83 (23.18%)], G1P [8] [27 (7.54%)] and G9P [4] [20 (5.59%)]. Mixed strains contribute a significant proportion of stool sample.
Rotavirus is an important cause of diarrhea in hospitalized children. There is continued circulation of G9 and G12 strains and the emergence of G3P [8] as most common strain.
估计 5 岁以下住院儿童轮状病毒腹泻的比例，确定循环的轮状病毒基因型，并了解轮状病毒疫苗对轮状病毒腹泻的流行和严重程度的影响。
这是一项在 29 个月（2017 年 9 月至 2020 年 1 月）期间进行的基于医院的横断面观察性研究。从年龄在 0-59 个月、患有急性腹泻并在急诊就诊或需要住院的儿童中采集粪便样本。采用酶联免疫吸附试验（ELISA）检测粪便样本中的轮状病毒，并采用已发表的方法对其进行基因分型。
在 1480 个样本中，360 个（24.32%）通过 ELISA 检测出轮状病毒阳性，其中大多数为男性（62.97%）。轮状病毒阳性率最高的年龄段为<11 个月（55.27%）。轮状病毒腹泻病例的腹泻发作次数和呕吐经历有统计学差异。冬季的发病率最高。最常见的 G 和 P 型组合是 G3P[8]株（122 株[34.08%]）、G2P[4]（83 株[23.18%]）、G1P[8]（27 株[7.54%]）和 G9P[4]（20 株[5.59%]）。混合株在粪便样本中占很大比例。
轮状病毒是住院儿童腹泻的重要原因。G9 和 G12 株仍在继续循环，G3P[8]作为最常见的株系出现。
