Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44511, Egypt.
Poultry Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt.
Poult Sci. 2023 Dec;102(12):103219. doi: 10.1016/j.psj.2023.103219. Epub 2023 Oct 20.
The present study aimed to investigate the efficacy of probiotics and prebiotics in controlling Escherichia coli (E. coli) spp. isolated from chicken. A total of 230 birds representing 19 different commercial breeds were taken from various points. Birds were monitored for postmortem and clinical investigation. Aseptically collected liver samples, lungs, kidneys, hearts, and yolk sacs were then subjected to bacterial isolation and identification. E. coli were observed in 9 pooled samples from 120 examined with an incidence of 7.5%. Nine farms were E. coli-positive, with an incidence of farm infection of 47.3%. The 9 suspected isolates of E. coli were profiled by morphological and microbiological identification of the colony, motility, and gram reaction. The serogroup analysis showed 9 different E. coli for which 3 other groups were identified: 2 E. coli O78, 3 E. coli O111, and 4 untyped groups. Nine isolates of E. coli were subjected to PCR. Molecular detection of 9 strains was conducted to find the virulence genes of E. coli strains (8 STX1, 4 STX2, and 9 EAE). Probiotics and prebiotics significantly increased the total erythrocytic and leukocytic counts throughout the experiment. The phagocytic percentage's main values at 14 d were 47 and 30%, respectively. An increase in the humoral immunity against Newcastle disease (ND) was noticed after ND vaccination. The geometric mean (HI) was 5.9 and 4.2 for probiotic and prebiotic, respectively. It could be concluded that probiotics and prebiotics could stimulate a nonspecific immune response against experimental infection with a virulent strain of E. coli spp.
本研究旨在探讨益生菌和益生元对控制鸡源大肠杆菌（Escherichia coli）的功效。从不同地点采集了代表 19 个不同商业品种的 230 只鸟类进行尸检和临床调查。无菌采集肝脏、肺脏、肾脏、心脏和卵黄囊样本，然后进行细菌分离和鉴定。从 120 个检查样本中观察到 9 个混合样本中存在大肠杆菌，发生率为 7.5%。9 个农场大肠杆菌呈阳性，农场感染率为 47.3%。通过对菌落形态和微生物学鉴定、运动性和革兰氏反应对 9 株疑似大肠杆菌分离株进行鉴定。血清群分析显示 9 株不同的大肠杆菌，其中另外 3 个组为：2 株大肠杆菌 O78、3 株大肠杆菌 O111 和 4 株未分型组。对 9 株大肠杆菌进行 PCR 检测。对 9 株大肠杆菌进行分子检测，以发现大肠杆菌菌株的毒力基因（8 株 STX1、4 株 STX2 和 9 株 EAE）。益生菌和益生元在整个实验过程中显著增加了总红细胞和白细胞计数。14 天时吞噬百分比的主要值分别为 47%和 30%。接种新城疫疫苗后，发现对新城疫的体液免疫有所增强。益生菌和益生元的几何平均值（HI）分别为 5.9 和 4.2。可以得出结论，益生菌和益生元可以刺激针对实验性感染大肠杆菌的非特异性免疫反应。
