Iritani Y, Sugimori G, Katagiri K
Avian Dis. 1977 Jan-Mar;21(1):1-8.
Serologic responses to Haemophilus gallinarum (HG) were compared in chickens artifically inoculated with living HG and vaccinated with HG bacterin, using tube agglutination (AGG), hemagglutination-inhibition (HI), and agar-gel precipitation (AGP) tests. Infected and vaccinated chickens differed markedly, as did infection routes early after treatment. HI response was delayed and lower in chickens infected intranasally than in vaccinated chickens. Both AGG and AGP responses peaked sooner than HI. AGP response was earlier in infected chickens, but later in vaccinated chickens. A maximum of three lines were observed. All which appeared for vaccinated chickens were similar to those for inoculated ones. A residual line remaining for very long periods for inoculated and vaccinated chickens appeared near the side of well of the HG antigen. The duration of symptoms following HG infection had no correlation with HI, AGG, and AGP titers. From these results, the HI test seems not to be the only serological diagnosis of HG infection in the field.
利用试管凝集试验（AGG）、血凝抑制试验（HI）和琼脂凝胶沉淀试验（AGP），比较了人工接种活鸡嗜血杆菌（HG）和接种HG菌苗的鸡对HG的血清学反应。感染鸡和接种疫苗的鸡有显著差异，治疗后早期的感染途径也不同。经鼻感染的鸡的HI反应比接种疫苗的鸡延迟且更低。AGG和AGP反应均比HI反应更早达到峰值。AGP反应在感染鸡中出现得更早，但在接种疫苗的鸡中出现得更晚。最多观察到三条沉淀线。接种疫苗的鸡出现的所有沉淀线与接种活菌的鸡相似。在HG抗原孔的一侧附近出现了一条在接种和接种疫苗的鸡中都能长期存在的残留沉淀线。HG感染后症状的持续时间与HI、AGG和AGP滴度无关。从这些结果来看，HI试验似乎不是现场诊断HG感染的唯一血清学方法。
