Wambura Philemon N
Department of Microbiology and Parasitology, Sokoine University of Agriculture, P O Box 3019, Morogoro, Tanzania.
Trop Anim Health Prod. 2010 Mar;42(3):483-6. doi: 10.1007/s11250-009-9447-z. Epub 2009 Sep 4.
The study was conducted to prepare and evaluate the use of autogenous vaccine from Avibacterium paragallinarum (strain Tan 1-05) in layer chickens. The results showed that all chickens vaccinated with autogenous vaccine with 10(8)CFU/mL in aluminum phosphate gel developed MAT antibodies (GMT of 2.8 log2 to 5.3 log2) against A. paragallinarum infection. Moreover, the results indicated that all chickens (n=6) selected from vaccinated chickens were protected against A. paragallinarum infection after challenge. No A. paragallinarum was isolated from these chickens. Nevertheless, all unvaccinated chickens did not develop antibodies, and all selected unvaccinated chickens (n=6) showed clinical signs consistent with infectious coryza (IC) where two of them died from the disease after challenge. The findings from the present and previous studies showed that the development of an inactivated IC vaccine from local strains if optimized and adopted may be the best possible way of controlling this economically important poultry disease.
本研究旨在制备并评估来自副鸡禽杆菌（Tan 1-05株）的自体疫苗在蛋鸡中的应用。结果显示，所有接种含10(8)CFU/mL副鸡禽杆菌自体疫苗于磷酸铝凝胶中的鸡只均产生了针对副鸡禽杆菌感染的微量凝集试验（MAT）抗体（几何平均滴度为2.8 log2至5.3 log2）。此外，结果表明，从接种疫苗的鸡只中挑选出的所有鸡只（n = 6）在攻毒后均受到保护，未感染副鸡禽杆菌。这些鸡只中未分离到副鸡禽杆菌。然而，所有未接种疫苗的鸡只均未产生抗体，且所有挑选出的未接种疫苗的鸡只（n = 6）均表现出与传染性鼻炎（IC）一致的临床症状，其中两只在攻毒后死于该病。本研究及先前研究的结果表明，如果优化并采用从本地菌株开发的灭活IC疫苗，可能是控制这种具有重要经济意义的家禽疾病的最佳途径。
